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A8887 Sigma-Aldrich

Acrylamide

suitable for electrophoresis, ≥99%

Synonym: 2-Propenamide, Acrylic acid amide

  • CAS Number 79-06-1

  • Linear Formula CH2=CHCONH2

  • Molecular Weight 71.08

  •  Beilstein/REAXYS Number 605349

  •  EC Number 201-173-7

  •  MDL number MFCD00008032

  •  eCl@ss 39031205

  •  PubChem Substance ID 24891378

  •  NACRES NB.22

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Properties

Related Categories Acrylamides, Core Bioreagents, Life Science Reagents for Western Blotting, Research Essentials
vapor density   2.45 (vs air)
vapor pressure   0.03 mmHg ( 40 °C)
assay   ≥99%
bp   125 °C/25 mmHg (lit.)
mp   82-86 °C (lit.)
solubility   water: soluble 2.5 g/10 mL, clear, colorless
suitability   suitable for electrophoresis
SMILES string   NC(=O)C=C
InChI   1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
InChI key   HRPVXLWXLXDGHG-UHFFFAOYSA-N

Description

General description

Acrylamide acts as a quencher of tryptophan fluorescence. Acrylamide alkylates SH groups of cysteine and ε-NH2 lysine residues of proteins during electrophoresis. Bis-acrylamide is required for crosslinking and acrylamide is vital for the polymerization of monomers. Acrylamide along with bis-acrylamide crosslinks to form polyacrylamide gels, which is used to separate protein and other biomolecules.

Acrylamide (AA) is a neurotoxin and carcinogen. Potato products like, French fries, potato chips and also several cereal-based products contains acrylamide. This highly reactive organic compound has the ability to polymerize to form polyacrylamide.

Application

Acrylamide has been used:
• for the synthesis of hydrogels
• for the preparation of polyacrylamide gel
• to agglomerate vimentin and keratin intermediate filament network

Acrylamide has been used:
• to agglomerate the vimentin and keratin intermediate filament network of K562cells
• in the preparation and characterization of soft culture substrate
• in hydrogel synthesis
• for determination of protein by sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) electrophoresis

Packaging

1 kg in glass bottle

100, 500 g in glass bottle

2.5 kg in poly drum

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 2074 6.1 / PGIII
WGK Germany 
WGK 3
RTECS 
AS3325000
Flash Point(F) 
280.4 °F - closed cup
Flash Point(C) 
138 °C - closed cup

Single- & multi-channel pipettes from BRAND
Protocols & Articles

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Peer-Reviewed Papers
15

References

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