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A9045 Sigma-Aldrich

Agarose, low gelling temperature

BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture

Synonym: 2-Hydroxyethyl agarose

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Properties

Related Categories Agarose, Cell Culture, Companion Products and Reagents, Core Bioreagents, Insect Platform,
Quality Level   200
product line   BioReagent
form   powder
application(s)   cell culture | insect: suitable
  cell culture | mammalian: suitable
  cell culture | plant: suitable
EEO   ≤0.1
mp   ≤65 °C ( at a 1.5% gel)
transition temp   congealing temperature 26-30 °C
gel strength   ≥200 g/cm2 (1% gel)
solubility   H2O: 10 mg/mL (with heat)
anion traces   sulfate (SO42-): ≤0.10%
foreign activity   RNase and DNase free

Description

General description

Agarose is an algal polysaccharide. Agarose is a thermoreversible, ion-dependent gelling agent. Agarose gel electrophoresis is useful for the clinical routine analyses of proteins in plasma and other body fluids. It is a low gelling temperature derivative with unique gelling properties. Gels forms at <30°C, remelt at temperatures in excess of 60°C. Gels exhibit excellent clarity and are particularly useful for the preparation of media containing heat-labile materials.

Application

Agarose, a low gelling temperature derivative, is the suitable reagent for:
• plant cell culture studies
• insect cell culture studies
• cell culture studies

It may be used for the following studies:
• Recovery of defined RNA and DNA fractions after electrophoretic separation.
• Cytochemical staining procedure to investigate the succinate dehydrogenase (SDH) activity in pre-ovulatory mouse oocytes.
• Purification of RNA in Caenorhabditis elegans by electrophoresis.

Packaging

5, 10, 25, 50, 100, 250 g in poly bottle

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I use Agarose, low gelling temperature?
This product forms a suspension in nearly any aqueous buffer. Heating will melt the agar, which, upon cooling, will form a gel.
Can Agarose, low gelling temperature, be autoclaved?
This agarose can be autoclaved.
What is the gel strength of Agarose, low gelling temperature?
We have a specification of not less than 200 g/cm2 for a 1.0% agarose gel.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles

Articles

Agarose Product Information

CAS Number: 9012-36-6 (product A9539) CAS Number: 39346-81-1 (products A9414, A4018, A6560, A9045, A0701) Synonyms: 3,6-Anhydro-α-L-galacto-β-D-galactan; FastLane agarose; Indubiose A4; NuSieve GTG; ...
Keywords: Affinity chromatography, Cell culture, Chromatography, Cloning, Counterimmunoelectrophoresis, Culture media, DNA electrophoresis, Diffusion, Electrophoresis, Gel electrophoresis, Immunodiffusion, Immunoelectrophoresis, Indicators, Ion Exchange, Isoelectric focusing, Melting, Molecular biology, Polymerase chain reaction, Purification, Sample preparations, Separation

Protocols

Gelling Agents - Plant Tissue Culture Protocol

Agar has long been used to solidify media for plant tissue culture. The type of agar or gelling agent used can influence the growth of the tissue in culture. Both purity and cost of the gelling agent...
Keywords: Cell culture, Centrifugation, Immobilization, Inductively coupled plasma, Reductions

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Agarose Selection Guide

Agarose Selection Guide – Click on the Product Number to Purchase Specialty Agarose – Click on the Product Number to Purchase
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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