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A9099 Sigma-Aldrich

Acrylamide

for molecular biology, ≥99% (HPLC)

Synonym: 2-Propenamide, Acrylic acid amide

  • CAS Number 79-06-1

  • Linear Formula CH2=CHCONH2

  • Molecular Weight 71.08

  •  Beilstein/REAXYS Number 605349

  •  EC Number 201-173-7

  •  MDL number MFCD00008032

  •  eCl@ss 39031205

  •  PubChem Substance ID 24891226

  •  NACRES NA.31

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Properties

Related Categories Acrylamide, Acrylamides, Core Bioreagents, Life Science Reagents for Protein Expression and Purification, Life Science Reagents for Western Blotting,
Quality Level   200
grade   for molecular biology
vapor density   2.45 (vs air)
vapor pressure   0.03 mmHg ( 40 °C)
assay   ≥99% (HPLC)
impurities   ≤0.005% Acrylic acid content
  ≤0.005% Water insolubles
conductivity   ≤10 μmho (35% solution)
bp   125 °C/25 mmHg (lit.)
mp   82-86 °C (lit.)
solubility   water: soluble 2.5 g/10 mL, clear, colorless
cation traces   Fe: ≤1 ppm
  Mg: ≤3 ppm
  heavy metals (as Pb): ≤5 ppm
foreign activity   DNase, RNase, protease, none detected
SMILES string   NC(=O)C=C
InChI   1S/C3H5NO/c1-2-3(4)5/h2H,1H2,(H2,4,5)
InChI key   HRPVXLWXLXDGHG-UHFFFAOYSA-N

Description

General description

Polyacrylamide gels are prepared using acrylamide and bis-acrylamide. Acrylamide is a water-soluble monomer, which forms a transparent stable insoluble gel. Bis-acrylamide is a cross-linking agent.

Application

Acrylamide has been used:
• in polyacrylamide gel electrophoresis
• in the preparation of cleared gel samples
• to determine the effect of L-cysteine on acrylamide-induced testicular toxicity

Packaging

1 kg in glass bottle

2.5 kg in poly bottle

25, 100, 500 g in glass bottle

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 2074 6.1 / PGIII
WGK Germany 
WGK 3
RTECS 
AS3325000
Flash Point(F) 
280.4 °F - closed cup
Flash Point(C) 
138 °C - closed cup

Single- & multi-channel pipettes from BRAND
Protocols & Articles

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Peer-Reviewed Papers
15

References

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