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A9104 Sigma-Aldrich

AKT3, active human

buffered aqueous glycerol solution, protein apparent mol wt ~83 kDa, ≥85% (SDS-PAGE), recombinant, expressed in E. coli

Synonym: PKBγ, Protein Kinase B γ, RAC-gamma serine/threonine-protein kinase, STK-2

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Properties

recombinant   expressed in E. coli
assay   ≥85% (SDS-PAGE)
form   buffered aqueous glycerol solution
specific activity   300-3000 units/mg protein
mol wt   protein apparent mol wt ~83 kDa
shipped in   dry ice
storage temp.   −70°C
Gene Information   human ... AKT3(10000)

Description

Application

Kinase activity is measured as the molar amount of phosphate incorporated Akt/SGK substrate peptide (RPRAATF), per minute per mg protein at 30 °C using a final concentration of 50 μM [32P]-ATP.

Biochem/physiol Actions

One of three highly conserved isoforms, which are designated in humans as PKBα (Akt1), PKBβ (Akt2), and PKBγ (Akt3). Akt3 is expressed widely, but not in liver or skeletal muscle, suggesting that its principle function may not be in regulating insulin signaling. Akt3 is involved in tumorigenesis and may contribute to the more aggressive clinical phenotype of the estrogen receptor-negative breast cancers and androgen-insensitive prostate carcinomas.

Unit Definition

One unit will incorporate 1 nanomole of phosphate into the AKT/SGK substrate peptide (RPRAATF) per minute at pH 7.2 at 30 deg C using a final concentration of 50 μm.

Physical form

Supplied at a concentration of approximately 0.1 mg/mL in 50 μl of 50 mM Tris-HCl, pH 7.5, 150 mM NaCl, 0.25 mM dithiothreitol (DTT), 0.1 mM EGTA and 30% glycerol

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles
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