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A9228 Sigma-Aldrich

Amyloglucosidase from Rhizopus sp.

≥40,000 units/g solid

Synonym: 1,4-α-D-Glucan glucohydrolase, Exo-1,4-α-glucosidase, Glucoamylase

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Properties

Related Categories 3.2.x.x Glycosidases, 3.x.x.x Hydrolases, Amylases, α-Glucosidase for Starch Hydrolysis (Amylose and Amylopectin) and Glycogen, Amyloglucosidase, Biochemicals and Reagents,
form   lyophilized solid
solubility   0.03 M sodium citrate-phosphate buffer, pH 4.5: soluble 5.0 mg/mL
storage temp.   −20°C

Description

Application

Amyloglucosidase is used to hydrolyze α-D-glucosides. It may be used in the brewing of beer and in the production of bread and juices. Amyloglucosidase, from Rhizopus sp., has been used to study the cleavage of oligosaccharides during ER-associated degradation of proteins (ERAD). The enzyme has been used in the glycosylation of N-vanillyl-nonanamide to form a water-soluble component with pharmacological applications. The glycogen was estimated in stipe residue of Coprinus cinereus by treating with amyloglucosidase and measuring the amount of glucose produced.

Packaging

1, 10 g in poly bottle

Biochem/physiol Actions

Amyloglucosidases are capable of hydrolyzing the α-D-(1-4), the α-D-(1-6), and the α-D-(1-3) glucosidic bonds of oligosaccharides .

Unit Definition

One unit will liberate 1.0 mg of glucose from starch in 3 min at pH 4.5 at 55 °C.

Physical form

Lyophilized salt free powder

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Protocols

Enzymatic Assay of Amyloglucosidase (EC 3.2.1.3)

This procedure may be used for the determination of Amyloglucosidase activity using starch as the substrate. The Spectrophotometric Stop Rate Determination [Absorbance at 340 nm (A340), Light path = ...
Keywords: Enzyme activity, Extinction coefficient

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Peer-Reviewed Papers
15

References

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