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A9414 Sigma-Aldrich

Agarose, low gelling temperature

BioReagent, for molecular biology

Synonym: 2-Hydroxyethyl agarose

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Properties

Related Categories Agarose, Agarose Gel, Core Bioreagents, Molecular Biology, Nucleic Acid Electrophoresis,
Quality Level   200
biological source   algae (red)
grade   for molecular biology
product line   BioReagent
form   powder
EEO   ≤0.10
mp   ≤65 °C
transition temp   congealing temperature 26-30 °C
gel strength   ≥200 g/cm2 (1% gel)
anion traces   sulfate (SO42-): ≤0.10%
foreign activity   DNase, RNase, NICKase, none detected

Description

General description

Agarose is a component of cell wall polysaccharides of marine red algae. It has high gelling property and is hydrophilic. It exists in random coil conformation.

Application

Agarose, low gelling temperature has been used:
• in the single cell gel electrophoresis of glioblastoma multiforme cells for genotoxicity studies
• in the preparation of collagen-agarose co-gels
• in the embedding of shrimp brain sections

Packaging

5, 10, 25, 50, 100, 250 g in poly bottle

Biochem/physiol Actions

Agarose is used in enzyme immobilization and electrophoresis. It is also used in food and cosmetic industry.

Analysis Note

The following is a list of properties associated with our agaroses:
Sulfate content - used as an indicator of purity, since sulfate is the major ionic group present.
Gel strength - the force that must be applied to a gel to cause it to fracture.
Gel point - the temperature at which an aqueous agarose solution forms a gel as it cools. Agarose solutions exhibit hysteresis in the liquid-to-gel transition - that is, their gel point is not the same as their melting temperature.
Electroendosmosis (EEO) - a movement of liquid through the gel. Anionic groups in an agarose gel are affixed to the matrix and cannot move, but dissociable counter cations can migrate toward the cathode in the matrix, giving rise to EEO. Since electrophoretic movement of biopolymers is usually toward the anode, EEO can disrupt separations because of internal convection.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Agarose Product Information

CAS Number: 9012-36-6 (product A9539) CAS Number: 39346-81-1 (products A9414, A4018, A6560, A9045, A0701) Synonyms: 3,6-Anhydro-α-L-galacto-β-D-galactan; FastLane agarose; Indubiose A4; NuSieve GTG; ...
Keywords: Affinity chromatography, Cell culture, Chromatography, Cloning, Counterimmunoelectrophoresis, Culture media, DNA electrophoresis, Diffusion, Electrophoresis, Gel electrophoresis, Immunodiffusion, Immunoelectrophoresis, Indicators, Ion Exchange, Isoelectric focusing, Melting, Molecular biology, Polymerase chain reaction, Purification, Sample preparations, Separation

Related Content

Agarose Selection Guide

Agarose Selection Guide – Click on the Product Number to Purchase Specialty Agarose – Click on the Product Number to Purchase
Keywords: Cell culture, Electrophoresis, Immunodiffusion, Immunoelectrophoresis, Molecular biology, Protein electrophoresis, Separation

Peer-Reviewed Papers
15

References

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