A9434 Sigma-Aldrich

Ammonium chloride

for molecular biology, suitable for cell culture, ≥99.5%

Synonym: Salmiac

  • CAS Number 12125-02-9

  • Linear Formula NH4Cl

  • Molecular Weight 53.49

  •  Beilstein Registry Number 4371014

  •  EC Number 235-186-4

  •  MDL number MFCD00011420

  •  eCl@ss 38050207

  •  PubChem Substance ID 24891452



Related Categories Ammonium Salts, Biochemicals, Buffers / Buffer salts, Chemical Synthesis, Core Bioreagents,
grade   for molecular biology
vapor density   1.9 (vs air)
vapor pressure   1 mmHg ( 160.4 °C)
assay   ≥99.5%
form   powder or crystals
mp   340 °C (subl.) (lit.)
anion traces   phosphate (PO43-): ≤2 ppm
  sulfate (SO42-): ≤20 ppm
cation traces   Fe: ≤2 ppm
  heavy metals (as Pb): ≤5 ppm
suitability   suitable for cell culture
foreign activity   DNase, RNase, protease, none detected



1 kg in poly bottle

500 g in poly bottle


Ammonium chloride has been used:
• as an inhibitor of endosome acidification in vero cells
• as an autophagy inhibitor in vascular smooth muscle cell (VSMC)
• in the depletion and lysis of erythrocytes in testis parenchyma homogenate

Biochem/physiol Actions

Ammonium chloride is one of the three principal components of the nitrogen cycle. Ammonium chloride administration promotes creatinine and urea clearance andinduces metabolic acidosis in mice. Excess of ammonium chloride inhibits Kreb′s pathway in brain and depletes ATP in astrocytes. Ammonium chloride inhibits acidification in the endosome-lysosome system. Ammonium chloride is a hemolysing agent used for the lysis of erythrocytes.

Safety & Documentation

Safety Information

GHS07  GHS07
Signal word 
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
UN 3077 9 / PGIII
WGK Germany 
Protocols & Articles


A general protocol for the generation of Nanobodies for structural biology

Els Pardon a, b , Toon Laeremans a, b , Sarah Triest a, b , Søren G F Rasmussen c , Alexandre Wohlkönig a, b , Armin Ruf d , Serge Muyldermans b, e , Wim G J Hol f , Brian K Kobilka g , Jan Steyaert ...
Keywords: AGE, Adsorption, Amplification, Antibiotics, Biochemistry, Biotinylations, Buffers, Cancer, Centrifugation, Chromatography, Cloning, Coagulation, Crystallization, DNA purification, Deaminations, Degradations, Detergents, Digestions, Diseases, Drug discovery, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Genetic, Genomics, Hematology, High performance liquid chromatography, Immobilization, Immunology, Infectious Diseases, Neuroscience, Nucleic acid annealing, Nucleic acid hybridization, Peptide synthesis, Phase transitions, Polymerase chain reaction, Purification, Sample preparations, Separation, Sequencing, Size-exclusion chromatography, Sterilizations, Transfection, Western blot, transformation

Experienced User Protocol | GenElut Mammalian Genomic DNA Miniprep Kit

For experienced users, an abbreviated protocol is listed below. For data, images, troubleshooting and other information, please see the full length protocol page.
Keywords: PAGE

GenElute Mammalian Genomic DNA Miniprep Kit Protocol

The GenElute™ Mammalian Genomic DNA Miniprep Kit provides a simple and convenient way to isolate pure genomic DNA from a variety of cultured cells, tissues (including rodent tails), and fresh whole b...
Keywords: AGE, Amplification, Anticoagulants, Cell disruption, Centrifugation, DNA purification, Degradations, Digestions, Electrophoresis, Evaporation, Gel electrophoresis, Molecular biology, Nucleic acid denaturation, Phase transitions, Polymerase chain reaction, Precipitation, Purification, Sample preparations, Sequencing

Peer-Reviewed Papers


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