A9477 Sigma-Aldrich


≥95% (HPLC)

Synonym: 11β,21-Dihydroxy-3,20-dioxo-4-pregnen-18-al, 11β,21-Dihydroxypregn-4-ene-3,18,20-trione, 18-Aldocorticosterone, Reichstein X

  • CAS Number 52-39-1

  • Empirical Formula (Hill Notation) C21H28O5

  • Molecular Weight 360.44

  •  Beilstein/REAXYS Number 3224996

  •  EC Number 200-139-9

  •  MDL number MFCD00051136

  •  PubChem Substance ID 24891458

  •  NACRES NA.77



Related Categories A-AM, Aldehydes, Bioactive Small Molecule Alphabetical Index, Bioactive Small Molecules, Building Blocks,
Quality Level   200
biological source   synthetic (organic)
assay   ≥95% (HPLC)
form   powder
application(s)   cell culture | mammalian: suitable
solubility   chloroform: 20 mg/mL, clear to slightly hazy, colorless to light yellow
shipped in   ambient
storage temp.   room temp
SMILES string   C[C@]12CCC(=O)C=C1CC[C@H]3[C@@H]4CC[C@H](C(=O)CO)[C@]4(C[C@H](O)[C@H]23)C=O
InChI   1S/C21H28O5/c1-20-7-6-13(24)8-12(20)2-3-14-15-4-5-16(18(26)10-22)21(15,11-23)9-17(25)19(14)20/h8,11,14-17,19,22,25H,2-7,9-10H2,1H3/t14-,15-,16+,17-,19+,20-,21+/m0/s1
Gene Information   human ... SERPINA6(866)
rat ... Ar(24208), Nr3c2(25672)



Aldosterone has been used:
• as a RPMI-1640 medium supplement for podocyte culture to test its pathogenicity in obesity-related glomerulopathy (ORG)
• in combination with salt to induce renal injury in mice
• to stimulate primary liver sinusoidal endothelial cells (LSECs) and in the generation of hyperaldosteronism model in mice

The d-isomer of aldosterone is considered to be the biologically active isomer.


100 mg in poly bottle

5, 25 mg in glass insert

Biochem/physiol Actions

Aldosterone is a biologically active aldosterone isomer; a mineralocorticoid produced by the adrenal cortex. It induces urinary excretion of K+ and renal reabsorption of Na+. It is produced from adrenocorticotropic hormone (ACTH) and is essential for maintaining sodium homeostasis in the distal tubules. Aldosterone binds to the mineralocorticoid receptor and may modulate vascular system and induce kidney damage. It contributes to the progression of chronic kidney disease(CKD). Aldosterone is part of the renin-angiotensin-aldosterone system (RAAS).

Other Notes

Exists as an equilibrium mixture of the aldehyde and the hemiacetal.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
IsProduct A9477, Aldosterone,the d- or l- isomer?
The A9477 is the d-Aldosterone (per our supplier), which is considered to be the biologically active isomer.
How do I make a stock solution of Product A9477, Aldosterone?
To prepare a 100 μg/ml stock solution, add 5 ml of absolute ethanol to 5 mg, gently swirl to dissolve, then add 45 ml sterile medium. Freeze the stock in working aliquots, but avoid repeated freeze/thaw cycles.
Is Product A9477, Aldosterone, tested for solubility?
Yes, A9477 is tested for solubility using 20mg/ml in chloroform. For biological applications, you can solubilize aldosterone in ethanol to at least 1 mg/mL. Please refer to literature references for further solubility information.
What is the difference between Aldosterone Products A9477 and 05521?
The main differences between A9477 and 05521 are the assays performed on the products. The assay results are lot specific and can be viewed on the Certificate of Analysis.
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706035 Aldosterone-2,2,4,6,6,21,21-d7 , ≥98 atom % D (based on d7), ≥98% (CP)

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