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A9731 Sigma-Aldrich

Albumin human

recombinant, expressed in rice, lyophilized powder, suitable for cell culture, low endotoxin, ≥96% (SDS-PAGE)

Synonym: Cellastim S, rHSA, rHSA

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Properties

Related Categories Albumin, Albumins and Transport Proteins, Bioactives and Supplements for Stem Cell Expansion, Biochemicals and Reagents, CHO Platform,
Quality Level   200
biological source   human
recombinant   expressed in rice
assay   ≥96% (SDS-PAGE)
form   lyophilized powder
mol wt   monomer ~67 kDa
application(s)   cell culture | mammalian: suitable
impurities   ≤1 EU/mg protein
storage temp.   2-8°C
Gene Information   human ... ALB(213)

Description

Application

This recombinant variant is recommended for use in absorption, distribution, metabolism, and excretion (ADME) pharmacological research; cell culture; drug delivery research; and cryopreservation of cells.

Packaging

1, 5, 10 g in poly bottle

Biochem/physiol Actions

Albumins are soluble monomeric proteins found in the body fluids and tissues of animals and in some plant seeds. Serum albumin functions as a carrier protein for steroids, fatty acids, and thyroid hormones. Serum albumins are also vital in regulating the colloidal osmotic pressures of blood.

Features and Benefits

Cellastim is a well defined, animal-free recombinant human albumin (rHA). Designed for cell culture applications, Cellastim is a powerful media supplement that outperforms plasma-derived human albumin, bovine serum albumin, and other sources of recombinant human albumin. Cellastim delivers consistent performance, not the variability associated with hydrolysates and extracts. Cellastim is ideally suited for use in cell culture media for Chinese Hamster Ovary (CHO), hybridoma, VERO, stem cells and primary cells.

Advantages
• Eliminates animal-derived additives
• Safe and regulatory friendly
• Proven effective in enhancing stem cell growth in embryonic, mesenchymal, neural, cardiac, liver, kidney, keratinocyte and fibroblast stem cell lines
• Maintains stem cells in an undifferentiated state
• Eliminates coating of culture plates for adherent cell lines
• Provides well-defined and consistent stem cell medium

Analysis Note

Assayed by SDS-PAGE

Other Notes

To view more information on Cellastim download the Cellastim Brochure, Cellastim CHO Poster, and Cellastim hybridoma Poster.

View more information on human serum albumin.

Legal Information

Cellastim is a trademark of InVitria

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Pipette Controller from Brand
Protocols & Articles

Articles

Lipid Induced Insulin Resistance

Obesity is a well-established risk factor for the development of insulin resistance. Obesity is associated with the increased deposition of lipids in non-adipose tissue with subsequent decreases in i...
Linda Stephenson, Ph.D.
Biofiles v6 n4, 9
Keywords: Angiogenesis, Antivirals, Apoptosis, Biological processes, Cardiovascular, Catalog, Catalysis, Cell culture, Cell proliferation, Deposition, Diabetes, Events, Gene expression, Growth factors, Hormones, Inflammation, Metabolism, Metabolites, Obesity, Phosphorylations, Physiological Processes, Reductions, Transcription, Transduction, Type

Related Content

Albumin in Cell Culture

Importance and uses of albumin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell cultures
Keywords: Cell culture, Culture media, Degradations, Hormones, Ligands, Oxidations, PAGE, Purification, Redox Reactions

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
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Peer-Reviewed Papers
15

References

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Description

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A8763 Albumin from human serum, lyophilized powder, essentially globulin free, ≥99% (agarose gel electrophoresis)

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