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A9891 Sigma-Aldrich

7-Amino-4-methylcoumarin

Chromophore for substrates

Synonym: Coumarin 120

  • CAS Number 26093-31-2

  • Empirical Formula (Hill Notation) C10H9NO2

  • Molecular Weight 175.18

  •  Beilstein/REAXYS Number 142231

  •  EC Number 247-454-8

  •  MDL number MFCD00006868

  •  PubChem Substance ID 24891406

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Properties

Related Categories Activity, Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Fluorogenic,
description   chromophore for enzyme substrates
InChI Key   GLNDAGDHSLMOKX-UHFFFAOYSA-N
mp   223-226 °C (lit.)
fluorescence   λex 365 nm; λem 440 nm in ethanol(lit.)
storage temp.   2-8°C

Description

Packaging

1 g in poly bottle

250, 500 mg in poly bottle

Application

Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.

Preparation Note

Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).

Other Notes

Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Sensor Dyes

In 1998 R.F. Furchgott, L.J. Ignarro, and F. Murad received the Nobel Prize in Physiology and Medicine for their discoveries concerning nitric oxide as a signaling molecule in the cardiovascular syst...
Monika Baeumle
BioFiles 2011, 6.3, 23.
Keywords: Applications, Biochemistry, Biological processes, Cancer, Cardiovascular, Central Nervous System, Diseases, Extinction coefficient, Indicators, Inflammation, Methods, Microscopy, Neurotransmission, Sol-gel, Type

Protocols

Enzymatic Assay of Cathepsin B

This procedure applies to all products that have a specification for Cathepsin B activity, such as Sigma-Aldrich Product Numbers C0150 and C8571, determined by the liberation of 7-amino-4-methylcouma...
Keywords: Enzyme activity, Enzymology

Related Content

Mass Spectrometry of Glycans | BioFiles Volume 5, Number 1 Glycobiology

Glycosylation is known to have profound influence on various physiochemical, cellular and biological functions of proteins. Alterations in this modification are known to affect the immune system and ...
Keywords: Addition reactions, Cancer, Capillary electrophoresis, Chromatography, Derivatizations, Digestions, Diseases, Electrophoresis, Eliminations, Fermentation, Filtration, Gas chromatography, Glycosylations, High performance liquid chromatography, Ion Exchange, Liquid chromatography mass spectrometry, Mass spectrometry, PAGE, Phase transitions, Proteomics, Purification, Sequencing

Peer-Reviewed Papers
15

References

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