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  • A9891 - 7-Amino-4-methylcoumarin

A9891 Sigma-Aldrich


Chromophore for substrates

Synonym: Coumarin 120

  • CAS Number 26093-31-2

  • Empirical Formula (Hill Notation) C10H9NO2

  • Molecular Weight 175.18

  •  Beilstein/REAXYS Number 142231

  •  EC Number 247-454-8

  •  MDL number MFCD00006868

  •  PubChem Substance ID 24891406

  •  NACRES NA.32



Related Categories Activity, Biochemicals and Reagents, Enzyme Substrates, Enzymes, Inhibitors, and Substrates, Fluorogenic,
description   chromophore for enzyme substrates
assay   ≥98% (HPLC)
form   powder
mp   223-226 °C (lit.)
solubility   acetone: 10 mg/mL, clear, colorless to yellow
fluorescence   λex 365 nm; λem 440 nm in ethanol(lit.)
storage temp.   2-8°C
SMILES string   CC1=CC(=O)Oc2cc(N)ccc12
InChI   1S/C10H9NO2/c1-6-4-10(12)13-9-5-7(11)2-3-8(6)9/h2-5H,11H2,1H3



7-Amino-4-methylcoumarin has been used:
• to determine cathepsin B like- activity
• as a substrate for leucine aminopeptidase (LAP)
• in chymotryptic assay
• as a standard to detect the activation of caspase-3 during
• sanguinarine-induced damage
• protection by human DEAD-box DDX3

Used as matrix for analysis of monosulfated disaccharides and as a co-matrix with 6-aza-2-thiothymidine for sulfated neutral and sialylated tri-and tetrasaccharides.


1 g in poly bottle

250, 500 mg in poly bottle

Biochem/physiol Actions

7-Amino-4-methylcoumarin is a coumarin derivative, which serves as a fluorescence reference standard to screen protease inhibitors.

Preparation Note

Soluble in DMSO (10 mg/mL), DMF, and acetone (10 mg/mL).

Other Notes

Reagent for preparing new fluorogenic AMC-based substrates for cystine aminopeptidase (and other hydrolases); used as reference compound in the enzyme assay.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


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Enzymatic Assay of Cathepsin B

This procedure applies to all products that have a specification for Cathepsin B activity, such as Sigma-Aldrich Product Numbers C0150 and C8571, determined by the liberation of 7-amino-4-methylcouma...
Keywords: Enzyme activity, Enzymology

Peer-Reviewed Papers


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