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A9975 Sigma-Aldrich

ADAM-10 from mouse

>90% (SDS-PAGE), recombinant, expressed in baculovirus infected Sf21 cells, lyophilized powder

Synonym: Kuzbanian, Mammalian disintegrin metalloproteinase, Myelin-associated metalloproteinase

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Properties

Related Categories Bioactives/Supplements, Cell Biology, Cell Signaling and Neuroscience, Cytoskeleton and Extracellular Matrix, Extracellular Matrix,
recombinant   expressed in baculovirus infected Sf21 cells
assay   >90% (SDS-PAGE)
form   lyophilized powder
mol wt   apparent mol wt 60 kDa by SDS-PAGE
  predicted mol wt 52 kDa
impurities   endotoxin, tested
storage temp.   −20°C
Gene Information   mouse ... Adam10(11487)

Description

Biochem/physiol Actions

ADAM-10 is a member of the ADAM family that contains a disintegrin and metalloprotease-like domain. Mouse, rat, bovine, and human ADAM10 share 97% amino acid identity.

Physical form

Lyophilized from a 0.2 μm filtered solution in 25 mM MES, 0.2 M sodium chloride, 5 mM calcium chloride, 2.5 mM zinc chloride, 0.005% Brij® L23, pH 5.0

Analysis Note

The bioactivity is measured in a bioassay using a coumarin peptide substrate and 100 ng enzyme.

Legal Information

Brij is a registered trademark of Croda International PLC

Safety & Documentation

Safety Information

Symbol 
GHS09  GHS09
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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