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A9981 Sigma-Aldrich

Monoclonal Anti-AP-2α antibody produced in mouse

~1.0 mg/mL, clone AP2a 8G8/5, purified immunoglobulin, buffered aqueous solution

Synonym: Anti-AP-2α, Anti-AP-2, Anti-AP2TF, Anti-TFAP2, Anti-TFAP2A

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Properties

Related Categories AA - CA, AO-AQ, Alphabetical Index, Antibodies, Antibodies for Epigenetics and Nuclear Signaling,
conjugate   unconjugated
clone   AP2a 8G8/5, monoclonal
biological source   mouse
application(s)   immunohistochemistry: suitable
  western blot: 1-2 μg/mL using SK-Mel 19 total cell extract
species reactivity   human
mol wt   antigen ~50 kDa
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG1
antibody product type   primary antibodies
concentration   ~1.0 mg/mL
UniProt accession no.   P05549
Gene Information   human ... TFAP2A(7020)

Description

General description

Adaptor protein-2 (AP2) is a key constituent of clathrin-coated vesicles that mediate the endocytosis of cell membrane components such as G protein-coupled receptors (GPCRs). AP2 is a heterotetramer that consists of α, β, μ and σ subunits which bind to tyrosine- and dileucine-based motifs on membrane-associated cargo proteins.
Mouse Monoclonal Anti-AP-2α antibody binds to human AP-2α.

Immunogen

human AP-2α/AP-2γ fusion protein.

Application

Immunoprecipitation and western blot analysis was performed on virally -infected A549 cells using monoclonal mouse anti-AP-2 IgG (clone 100/2).

Mouse Monoclonal Anti-AP-2α antibody can be used for western blot (1-2μg/ml) and IHC assays.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
nwg

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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A7107 Monoclonal Anti-AP2 antibody produced in mouse, ~1.0 mg/mL, clone A6/2/2, purified immunoglobulin, buffered aqueous solution

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