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  • ABK0002 - ErbiBridge ELISA Kit

ABK0002 Sigma-Aldrich

ErbiBridge ELISA Kit

  •  NACRES NA.32



Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
antibody form   IgG fraction of antiserum
species reactivity   human (Human IgG)
shipped in   wet ice
storage temp.   2-8°C


General description

A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full length protein sequence of Cetuximab. Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of many of them is masked by the three dimensional structure of the protein and only a limited number of the antibodies respond. The result is a histogram which can be likened to a ‘fingerprint’ for correctly folded Cetuximab. For an Erbitux Biosimilar, if the protein is correctly folded and glycosylated, the ‘fingerprint’ will match that of Cetuximab. If it is not correctly folded, previously masked peptide sequences will be exposed and will be recognized by the antibody made to that exposed sequence. In this way, changes in the ‘fingerprint’ generated by the ELISA will point out differences between the Biosimilar and authentic Cetuximab.


For research use only. Not for use in diagnostic procedures.

Safety & Documentation

Safety Information

GHS05  GHS05
Signal word 
Hazard statements 
Precautionary statements 
UN 3316 9


Certificate of Analysis (COA)

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Protocols & Articles


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ErbiBridge ELISA Kit Protocol

Complete kit for the systematic 3-D conformational comparability analysis of Erbitux biosimilar molecule to Cetuximab (Erbitux trade name). Read the entire protocol before performing the assay. This ...
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