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  • ABK0015 - GoliBridge ELISA Kit from rabbit serum

ABK0015 Sigma-Aldrich

GoliBridge ELISA Kit from rabbit serum

  •  NACRES NA.32



Related Categories Antibodies, Antibody Kits, ELISA Kits, Sandwich ELISAs
antibody form   purified immunoglobulin
species reactivity   human (IgG)
shipped in   wet ice
storage temp.   2-8°C


General description

A Protein Conformational Array (PCA) in a sandwich ELISA format where the plate is coated with a panel of antibodies raised against peptides derived from the full length protein sequence of Golimumab. Taken individually, each of these antibodies is strongly antigenic to the peptide sequence that was used in its production. However, when these peptides are incorporated into a full length correctly folded protein, the antigenicity of many of them is masked by the three dimensional structure of the protein and only a limited number of the antibodies respond. The result is a histogram which can be likened to a ‘fingerprint’ for correctly folded Golimumab. For a Simponi Biosimilar, if the protein is correctly folded and glycosylated, the ‘fingerprint’ will match that of Golimumab. If it is not correctly folded, previously masked peptide sequences will be exposed and will be recognized by the antibody made to that exposed sequence. In this way, changes in the ‘fingerprint’ generated by the ELISA will point out differences between the Biosimilar and authentic Golimumab.

Other Notes

ELISA kit contains 3 96-well microplates and all the components necessary to complete an ELISA test.

Safety & Documentation

Safety Information

UN 2796 8 / PGII


Certificate of Analysis (COA)

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Protocols & Articles


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GoliBridge ELISA Kit Protocol

Complete kit for the systematic 3-D conformational comparability analysis of Simponi biosimilar molecule to Golimumab (Simponi trade name).
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