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  • AC-0350 - Neuron-specific enolase (NSE) (EP319) Rabbit Monoclonal Primary Antibody

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AC-0350 Sigma-Aldrich

Neuron-specific enolase (NSE) (EP319) Rabbit Monoclonal Primary Antibody

  •  NACRES NA.41

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Properties

biological source   rabbit
antibody form   Ig fraction of antiserum
clone   monoclonal
description   For In Vitro Diagnostic Use in Select Regions (See Chart)
form   buffered aqueous solution
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:20
shipped in   wet ice
storage temp.   2-8°C
visualization   cytoplasmic

Description

General description

Neuron-specific enolase (NSE), also known as enolase 2 (ENO2), is a 78 kDa gamma-enolase homodimer and one of three mammalian isozymes catalyzing the interconversion of 2-phosphoglycerate and phosphoenolpyruvate. The C-terminus promotes cell survival by regulating neuronal growth factor receptor-dependent signaling pathways. NSE localizes in the cytosol and is expressed in mature neurons and throughout the neuroendocrine system, predominantly in cells of the amine precursor uptake and decarboxylation lineage. Literature reports showed low expression of NSE in other cell types, where it may be located on the membrane and have non-glycolytic functions. These include erythrocyte, platelet, prostate, uterine, breast, striated muscle, and smooth muscle cells. However, this antibody specifically labels neuronendocrine cells in normal tissues. NSE is the most sensitive immunohistochemical marker for middle to late stage small cell lung cancer (SCLC) and can also identify Merkel cells and melanocytes. NSE overexpression is primarily found in tumors of neurogenic and neuroendocrine origin. Incidences of normally NSE-negative cells producing NSE have been found in malignant transformations; thus, NSE should be paired with the more specific bombesin, which does not stain non-neuroendocrine cells, and/or chromogranin, which intensely stains lung carcinoid tumors. Serum NSE is also useful in identifying small-cell lung carcinoma (SCLC), carcinoids, islet cell tumors and neuroblastomas, and are potential indicators of neural injury, such as reactive gliosis, astrocytic death, and blood-brain barrier dysfunction.

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Physical form

Solution in Tris Buffer, pH 7.3-7.7, with 1% BSA and &< 0.1% Sodium Azide

Other Notes

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Safety & Documentation

Safety Information

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Documents

Certificate of Analysis (COA)

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