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AEC101 Sigma-Aldrich

AEC Staining Kit

liquid

  •  NACRES NA.32

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Description

Application

AEC Staining Kit has been used as a substrate
• for developing the extravidin peroxidase antibody in cell-seeded matrice sections using histology and immunohistochemistry methods
• for streptavidin-horseradish peroxidase in peripheral blood mononuclear cells in ex vivo enzyme-linked immunospot (ELISPOT) assay
• for mouse IgG biotinylated secondary antibody in immunohistostaining of epididymal fat pads

Packaging

Kit contains 3 mL of concentrated acetate buffer, 3-amino-9-ethylcarbazole (AEC) chromogen and hydrogen peroxide in a dropper bottle for easy dispensing. Directions for use with each application are included.

Caution

Stable at least one year at 2-8 °C.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
RIDADR 
UN 3316 9

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Single- & multi-channel pipettes from BRAND
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Immunohistochemistry Protocol

Immunohistochemical staining is a valuable tool for detecting specific antigens in tissues. In order to perform the standard staining procedure, first the tissue section has to be deparaffinized and ...
Keywords: Digestions, Immunohistochemistry, Solvents

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Peer-Reviewed Papers
15

References

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