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  • APOAC - Annexin V-Cy3 Apoptosis Detection Kit

APOAC Sigma-Aldrich

Annexin V-Cy3 Apoptosis Detection Kit

  •  NACRES NA.32



Features and Benefits

Detects apoptosis earlier in the process than DNA-based assays such as TUNEL
• Rapid labeling of cells. Cell staining takes only 10 minutes.
• No cell fixation or processing required, reducing the detection time and allowing the cells to be used for further study.
• 6-Carboxyfluorescein diacetate secondary dye is included with the kit to differentiate apoptotic cells from viable and necrotic cells.


Sigma′s Annexin V-Cy3 kit allows detection of annexin V bound to apoptotic cells by fluorescence microscopy. The Annexin V-Cy3 kit uses the dye Cy3.18 as the fluorochrome conjugated with annexin V. By microscopy, Cy3.18 fluoresces more brightly than the FITC conjugate. The kit includes the non-fluorescent compound 6-carboxyfluorescein diacetate (6-CFDA), which enters the cell and is hydrolyzed by the esterases present in living cells to the fluorescent compound 6-carboxyfluorescein, indicating that the cells are viable. This combination allows the differentiation among early apoptotic cells (annexin V positive, 6-CFDA positive), necrotic cells (annexin V positive, 6-CFDA negative), and viable cells (annexin V negative, 6-CFDA positive).

Legal Information

Cy3 is a trademark of GE Healthcare

Kit component only


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10× Binding buffer 20 mL    

Standard component


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6-Carboxyfluorescein diacetate 10 mg SDS C5041
Annexin V-Cy3.18 Conjugate from human placenta 10 μg SDS A4963
Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Protocols & Articles


Apoptosis Assays

Apoptosis, or programmed cell death, is a growth-limiting regulatory mechanism by which cells can trigger their own death in response to extracellular signals because of irreparable cellular or DNA d...
Keywords: Apoptosis, Autophagy, Cancer, Cell culture, Detection methods, Diseases, Enzyme-linked immunosorbent assay, Flow cytometry, Immunofluorescence, Immunohistochemistry, Microscopy, Phosphorylations

Peer-Reviewed Papers


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