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AV30005 Sigma-Aldrich

Anti-IKBKG antibody produced in rabbit

IgG fraction of antiserum

Synonym: Anti-Inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase γ

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Properties

Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Gene Regulation and Expression, Antibodies for Kinase/Phosphatase Biology,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   western blot: suitable
species reactivity   horse, rabbit, human, dog
mol wt   mol wt 48 kDa
form   buffered aqueous solution
shipped in   wet ice
storage temp.   −20°C
antibody form   IgG fraction of antiserum
antibody product type   primary antibodies
concentration   0.5 mg - 1 mg/mL
NCBI accession no.   NP_001093326
UniProt accession no.   Q9Y6K9
Gene Information   human ... IKBKG(8517)

Description

Immunogen

Synthetic peptide directed towards the middle region of human IKBKG

Application

Anti-IKBKG antibody produced in rabbit is suitable for western blotting at a concentration of 1.25 μg/ml.

Physical form

Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% sucrose.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

The Inhibitor of nuclear factor-κB (IκB) kinase (IKK) complex is a central regulator of NF-κB signaling and activation of target genes. The IKK complex is made up of IKK1, IKK2 and NEMO or IKK γ. Two subunits of NEMO bind the IKK1-IKK2 heterodimer to make up the IKK complex. IKK γ lacks a catalytic domain but has regulatory function on the IKK complex.

Sequence

Synthetic peptide located within the following region: LGELQESQSRLEAATKECQALEGRARAASEQARQLESEREALQQQHSVQV

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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