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B0631 Sigma-Aldrich

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt

≥90%

Synonym: 5-BrdUTP

  • CAS Number 102212-99-7

  • Empirical Formula (Hill Notation) C9H14BrN2O14P3

  • Molecular Weight 547.04

  •  MDL number MFCD00057405

  •  PubChem Substance ID 24891527

  •  NACRES NA.51

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Properties

Related Categories Biochemicals and Reagents, Nucleosides, Nucleotides, Oligonucleotides, Nucleotide Analogs More...
biological source   synthetic (organic)
assay   ≥90%
form   powder
solubility   water: 50 mg/mL, clear, colorless to faintly yellow
storage temp.   −20°C
SMILES string   [Na].OC1CC(OC1COP(O)(=O)OP(O)(=O)OP(O)(O)=O)N2C=C(Br)C(=O)NC2=O
InChI   1S/C9H14BrN2O14P3.Na.H/c10-4-2-12(9(15)11-8(4)14)7-1-5(13)6(24-7)3-23-28(19,20)26-29(21,22)25-27(16,17)18;;/h2,5-7,13H,1,3H2,(H,19,20)(H,21,22)(H,11,14,15)(H2,16,17,18);;
InChI key   UWZGQOWZUSBZMC-UHFFFAOYSA-N

Description

General description

5-Bromo-2′-deoxyuridine 5′-triphosphate is used for incorporation into DNA for studying normal and tumor cell proliferation profiles.

Application

5-Bromo-2′-deoxyuridine 5′-triphosphate (5-BrdUTP) may be used as a labeling TdT substrate for terminal deoxynucleotidyl transferase (TdT) reactions or for DNA synthesis. BrdUTP is also used as an effective mutagenic agent. 5-BrdUTP labeling may be detected by immunological techniques or 5-BrdUTP may be derivitized by biotinylation or other methods for detection by fluorogenic or chromogenic methods.

5-Bromo-2′-deoxyuridine 5′-triphosphate sodium salt has been used:
• in the labelling of 3′-OH termini of fragmented DNA with double stranded breaks in african green monkey kidney cells
• to label cleaved DNA ends in apoptosis assay in avian tissue sections
• for incorporation into mice prostates for immunohistochemistry studies

Packaging

1, 5 mg in glass bottle

25 mg in poly bottle

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Target organs 
Respiratory system
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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