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B11000 Sigma

EnPresso® B

Growth system for expressing protein in bacteria, Starter Pack

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Properties

Related Categories Bacterial Culture Media, Cell Biology, EnPresso Growth Systems for microbial culture, Life Science Research, Microbial Media for Molecular Biology and Protein Expression,
sterility   sterile; γ-irradiated
form   tablet
pH   6.8
shipped in   ambient
storage temp.   room temp

Description

Biochem/physiol Actions

EnPresso B is a pre-sterilized growth system designed to increase the yield of functional protein from E. coli-based expression systems.

EnPresso growth systems provide optimal conditions for growth, metabolism and protein expression in microbial cultures. Protein yields are increased by enabling cultures to reach far higher cell densities than those achieved using conventional media. By controlling growth rate and metabolism, a greater proportion of expressed protein can be correctly folded to improve solubility, minimize the risk of inclusion body formation, and ensure functionality of the final product.

EnPresso growth systems maintain pH, provide adequate minerals, vitamins and trace elements to support growth, and use proprietary EnBase technology to ensure a constant, slow release of glucose from a polysaccharide substrate.

See all available products from EnPresso B Growth Systems.

Physical form

EnPresso® B is supplied in a kit providing sufficient reagents for 4 separate 50ml cultures. Included in the kit:
8 tablets in 4 white bags
4 tablets in 4 black bags
1 bottle (1 ml) Reagent A

Legal Information

EnPresso is a registered trademark of BioSilta Oy

Other Notes

BioSilta performance guarantee for EnPresso Starter Packs:
For expression of recombinant proteins in E. coli, BioSilta guarantees a minimun 5-fold increase in protein yield from EnPresso B or EnPresso B animal-free growth systems when compared to yields from comercially available growth media. Comparisons must be made using an EnPresso Starter Pack and a commercially-available growth media. Manufacturer′s protocols must be followed exactly. Cultures must be adequately aerated in shake flasks or 24 deepwell plates. Failure to achieve minimum 5-fold increase in yield must be reported to BioSilta (through Sigma Technical Services) within 21 days of product delivery and include quantitative data from the EnPresso growth system and the comparative growth media. Subject to data review, BioSilta/Sigma will refund the purchase price of the EnPresso Starter Pack. Please note this guarantee does not apply to membrane proteins and proteins expressed into the periplasm as performance data is not yet available.

Safety & Documentation

Safety Information

Symbol 
Signal word 
Danger
Hazard statements 
RIDADR 
UN 3077 9 / PGIII
WGK Germany 
3

Documents

Certificate of Analysis


Milli-Q® Water Purification Solutions
Protocols & Articles

Articles

Biochemical and Structural Characterization of LiaR from Vancomycin-Resistant E. faecalis: the 'Master Regulator' of the Cell-Envelope Stress Response

The CDC estimates there are over 66,000 enterococcus infections per year in the United States (CDC, 2013) and has classified vancomycin resistant enterococci (VRE) as a serious threat. In the absence...
Milya G. Davlieva1, Yiwen Shi1, Michael Zianni2, Troy Johnson3, Paul Leonard3, John Ladbury3, Cesar A. Arias4,5 and Yousif Shamoo1.
Keywords: Antibiotics, Antimicrobials, Clinical, Gene expression, Phosphorylations, Purification

EnBase Technology - how it works (with webinar)

EnBase technology is a key element within every EnPresso® growth system, offering a novel approach to control the feeding, and thereby growth, of microbial cultures. A glucose-releasing agent breaks ...
Keywords: Deaminations, Metabolism, Metabolites, Respiration

EnPresso FAQs

A. EnPresso B growth system ready for use. Note small crystals of magnesium salts are visible – these do not affect performance.
Keywords: Anaerobic, Antibiotics, Cell disruption, Degradations, Gene expression, Indicators, Metabolism, Nutrition, Protein extraction, Purification, Reductions, Respiratory, Tagged proteins

EnPresso® B Growth Systems (E. coli protein data)

EnPresso(TM) B Growth Systems have been used to express a variety of protein types in a variety of different cells. The data on the chart below summarizes the proteins and cells used by our customers...
Keywords: Antibiotics, Gene expression, Genetic, Pharmaceutical

EnPresso® Growth Systems: Revolutionizing the Role of Microbial Cell Culture

Growing cells to express proteins may seem just a small step on the way to achieving your research goals, but…
Keywords: Cell culture, Metabolites

Introduction to Microbial Media

Microbiological studies require the availability of different species of bacteria, yeast or viruses in the laboratory. Large scale processes such as fermentation, protein and vaccine production requi...
Keywords: Antibiotics, Cellular processes, Cloning, Culture media, Fermentation, Gene expression, Growth factors, Indicators, Molecular biology, Nutrition, Sterilizations, Vitamins, transformation

NitroGENIUS: Engineering E. coli to Fix Nitrogen and Regulating Transcription with Light

Sigma-Aldrich congratulates Team NitroGENIUS on their Silver Medal at iGEM in Boston, 2014. The International Genetically Engineered Machine competition (iGEM) is the premiere undergraduate Synthetic...
Benjamin Huang1, Caroline Focht1, Hongyi Richard Li1, Jeffrey Lee1, Andrew Ng2, Bertram Berla3, Cheryl Immethun3, Deng Liu2, Tae Seok Moon3, and Himadri B. Pakrasi2,3
iGEM, Washington University in St. Louis1 Dept. of Biology, Washington University in St. Louis2 Dept. of Energy, Environmental and Chemical Engineering, Washington University in St. Louis3
Keywords: Anaerobic, Gas chromatography, Gene expression, Photosynthesis, Poisons, Reductions, Transcription

Protein Expression Systems

The development of genetic engineering and cloning has opened many possibilities of expression and isolation of heterologous proteins for research purposes. Considerable advances in technology have e...
Keywords: Amplification, Antibiotics, Cell disruption, Cloning, Culture media, Gene expression, Genetic, Genetics, Glycosylations, Molecular biology, Purification, Pyrogens, Transfection, transformation

Protocols

EnPresso® B Protocol with Video

EnPresso B is a pre-sterilized growth system designed to increase the yield of functional protein from E. coli-based expression systems. EnPresso growth systems provide optimal conditions for growth,...
Keywords: Antibiotics, Diagnostic, Filtration, Gene expression, Metabolism, Vitamins

Related Content

Synthetic Biology Resources

Sigma® Life Science is working to use our stregnths to build the field of Synthetic Biology. Below is a collection of links to our favorite resources. Sigma also offers thousands of products for your...
Keywords: Cell culture, Cloning, Gene expression, Genetic, Genetics, Metabolic Pathways, Metabolites, Molecular biology, Molecular biology techniques, Photosynthesis, Poisons, Polymerase chain reaction - quantitative, Transcription, Transfection

Webinar: High Protein Yields in Shaken Cultures by EnPresso® Growth System

EnPresso, a proprietary growth system from BioSilta Ltd, applies slow enzymatic glucose release to control the growth and protein synthesis rate in bacteria and yeasts. This high-cell-density growth ...
Keywords: Gene expression, Molecular biology

Peer-Reviewed Papers
15

References

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