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B1155 Sigma-Aldrich

bisBenzimide H 33258

powder, BioReagent, suitable for cell culture, ≥98% (HPLC and TLC)

Synonym: 2′-(4-Hydroxyphenyl)-5-(4-methyl-1-piperazinyl)-2,5′-bi(1H-benzimidazole) trihydrochloride, 2-[2-(4-Hydroxyphenyl)-6-benzimidazoyl]-6-(1-methyl-4-piperazyl)benzimidazole trihydrochloride, BBIH, BXI-72, HOE 33258, Hoechst 33258, NSC334072

  • CAS Number 23491-45-4

  • Empirical Formula (Hill Notation) C25H24N6O · 3HCl

  • Molecular Weight 533.88

  •  Beilstein/REAXYS Number 4088183

  •  EC Number 245-690-6

  •  MDL number MFCD00012679

  •  PubChem Substance ID 24891565

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Properties

Related Categories Alphabetical, Apoptosis Detection, Apoptosis and Cell Cycle, B, Bioactive Small Molecule Alphabetical Index,
InChI Key   OWRSPPSBNWJJAR-UHFFFAOYSA-N
product line   BioReagent
assay   ≥98% (HPLC and TLC)
form   powder
solubility   H2O: 10 mg/mL
  water and ethanol: 10 mg/mL
suitability   passes application test for fluorescence
  suitable for cell culture
storage temp.   −20°C

Description

Application

bisBenzimide H 33258 is useful for staining DNA, chromosomes and nuclei. bisBenzimide H 33258 may be used for fluorescence microscopy or flow cytometry.
Excitation max. = 346 nm
Emission max. = 460 nm

Packaging

100 mg in glass bottle

25 mg in poly bottle

Biochem/physiol Actions

bisBenzimide H 33258 is a potent and selective Bcl-XL inhibitor. bisBenzimide H 33258 is a membrane-permeable, fluorescent DNA stain with low cytotoxicity that intercalate in A-T regions of DNA.

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
SM1140500

Documents

Certificate of Analysis (COA)

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Pipette Controller from Brand

Cell Culture Guide
Protocols & Articles

Articles

Mycoplasma Detection/Elimination

The maintenance of contamination-free cell lines is essential to cell-based research. Among the biggest concerns are mycoplasma contamination. Although mycoplasma do not usually kill contaminated cel...
Don Finley
BioFiles v7 n1, 2011, 14–15
Keywords: Eliminations, Metabolism, Polymerase chain reaction

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Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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