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B2025 Sigma-Aldrich

Brilliant Blue G - Colloidal Concentrate

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Description

General description

Coomassie Brilliant Blue stain is widely used for the detection of proteins in sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS PAGE). Staining with brilliant blue G is called as blue silver staining due to its improved sensitivity close to silver staining method. Coomassie Brilliant Blue G stain associates with proteins via hydrophobic interaction. It is effective in staining anterior capsule in cornea with relatively less toxic effects.

Application

Brilliant Blue G - Colloidal Concentrate has been used in the staining
• protein fragments post in vitro pepsin digestion
• ovarian tumor fluid proteome prior to profiling
• the transcription factor (TFIID) complex from Saccharomyces cerevisiae

Packaging

1 ea in poly bottle

Reconstitution

The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 liter with 800 mL water.

Analysis Note

This product has been tested for suitability on SDS-PAGE.

Safety & Documentation

Safety Information

Symbol 
GHS05  GHS05
Signal word 
Warning
Hazard statements 
Precautionary statements 
RIDADR 
UN 1805 8 / PGIII
WGK Germany 
2

Documents

Certificate of Analysis

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How would you recommend that I reconstitute Product B2025, Brilliant Blue G?
One bottle of the productmakes 1 liter of working solution.The suspension will contain 0.1% (w/v) Brilliant Blue G, 0.29 M phosphoric acid and 16% saturated ammonium sulfate after diluting to 1 Liter with 800 mL water.
What is the solution stability of Product B2025, Brilliant Blue G?
Once diluted the solution should be stable indefinitely.It should be stored in the refrigerator and shaken well before each use.The solution tends to precipitate on standing and heat accelerates precipitation.Therefore, do not leave the bottle at room temperature overnight.
Can you use Product B2025, Brilliant Blue G, with nitrocellulose?
Use with nitrocellulose is not recommended, due to the methanol.
How is Product B2025, Brilliant Blue G, better than other Coomassie Blue dyes?
The major advantage of the colloidal form is that it gives less background staining that the "regular" non-colloidal Coomassie Brilliant Blue G and Brilliant Blue R stains. Sensitivity can be markedly increased by double staining, first with Coomassie Brilliant Blue G Colloidal, then Rapid Silver Stain.
What is the detection limit of Product B2025, Brilliant Blue G?
The detection limit for proteins is about 50 ng of protein. However, proteins pick up stains differently so this is a general description and may not apply to all proteins
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
My question is not addressed here, how can I contact Technical Service for assistance?
Ask a Scientist here.
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Protocols & Articles

Protocols

Brilliant Blue G – Colloidal Concentrate Protocol

Brilliant Blue G-Colloidal Concentrate has been designed for post-electrophoresis staining of proteins in IEF, PAGE, and SDS-PAGE gels. Fixing the proteins prior to staining is recommended for maximu...
Keywords: Electrophoresis, Isoelectric focusing, PAGE

Peer-Reviewed Papers
15

References

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