• USA Home
  • B2518 - Albumin, Acetylated from bovine serum

EMAIL THIS PAGE TO A FRIEND
B2518 Sigma-Aldrich

Albumin, Acetylated from bovine serum

lyophilized powder, suitable for microbiology

Synonym: Ac-BSA, Acetyl BSA, Acetylated BSA

Purchase

Properties

Related Categories Albumin, Albumins, Albumins by Application, Biochemicals and Reagents, Blocking Reagents for Hybridization,
Quality Level   200
biological source   bovine
form   lyophilized powder
purified by   heat shock fractionation
origin   USA origin
application(s)   Southern blotting: suitable
  blocking: suitable
impurities   HIV I and HIVII, HCV and HBsAg, tested negative
pH   7
suitability   suitable for microbiology
foreign activity   DNase free
  DNase, RNase, NICKase, protease, none detected
  NICKase free
  Protease free
  RNase free
storage temp.   2-8°C
Gene Information   bovine ... ALB(280717)

Description

General description

Native bovine serum albumin (BSA) is a 66 kDa protein, which belongs to the family of serum albumin. It consists of three domains (I, II and III) and is the major protein found in circulatory system. Native BSA is an α-helical, globular and non-glycosylated protein, with 17-disulfide bonds.

Acetylation of bovine serum albumin modifies various residues, most notably lysine (Lys), and also serine (Ser) and threonine (Thr) to lesser extents. MALDI-TOF mass spectrometry studies on acetylated BSA have indicated a MW value about 3000 Da higher compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. Acetylation of BSA also modifies parallel amino acid residues in any trace contaminating nucleases in the BSA, which inactivates that trace nuclease content. Thus acetylated BSA can be used in molecular biology applications where trace nuclease activity must be minimized.

Application

Select the right albumin for your application with the Albumin Selection Guide

This B2518 acetylated BSA product has been cited in various publications and protocols, such as the following:
* As an electrophoresis standard: Lindberg, T. et al., J. Pediatr. Gastroenterol. Nutr., 27(1), 30-36 (1998)
* In in situ end-labeling buffer for labeling of DNA fragments: Save, V. et al., ”Detecting and Quantifying Apoptosis in Tissue Sections”, in Apoptosis Methods and Protocols (H.J.M. Brady, ed.). Methods Mol. Biol., Vol. 282, pp. 67-84 (2004)
* In EMSA studies on nucleocytoplasmic shuttling: Ghosh, C.C. et al., “Analysis of Nucleocytoplasmic Shuttling of NF?B Proteins in Human Leukocytes”, in Membrane Trafficking (A. Vancura, ed.). Methods Mol. Biol., Vol. 457, pp. 279-292 (2008)
* In selection buffer for ribosome display studies: Larman, H.B. et al., Nat. Protoc., 9(1), 90-103 (2014)

Acetylated bovine serum albumin has been used:
• as a component of protease buffer for protease inhibition assay
• as a blocking agent in the preparation of cDNA glass array
• to select hybridoma antibodies sensitive to M-ficolin epitopes by immunofluorometric assay

Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

Packaging

100, 500 mg in glass bottle

Biochem/physiol Actions

Native bovine serum albumin (BSA) acts as a transporter of drugs, hormones and fatty acids. It has calcium binding sites and regulates metal homeostasis.

Features and Benefits

• Acetylated to inactivate trace nucleases
• DNase free / Exonuclease free
• Nickase free / Endonuclease free
• RNase free
• Protease free

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Albumin from Bovine Serum Product Information

Albumins bind, sequester and stabilize a range of important molecules and proteins. Bovine serum albumin (BSA) is a small (~66 kDa) globular albumin protein that has been utilized in many well-cited ...
Keywords: Affinity chromatography, Cell culture, Chromatin immunoprecipitation, Chromatography, Crystallization, Dialysis, Diffusion, Electrophoresis, Enzyme-linked immunosorbent assay, Fractionation, Hormones, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunoprecipitation, Ion Exchange, Nucleic acid hybridization, Precipitation, Purification, Sequencing, Western blot

Peer-Reviewed Papers
15

References

Related Products

related product

Product #

Description

Add to Cart

08168 Timestrip Plus 8 °C

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?