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B2904 Sigma-Aldrich

Bluo-Gal

reagent for selection of recombinant bacterial clones

Synonym: 5-Bromo-3-indolyl β-D-galactopyranoside, Bluo-Gal

  • CAS Number 97753-82-7

  • Empirical Formula (Hill Notation) C14H16BrNO6

  • Molecular Weight 374.18

  •  Beilstein/REAXYS Number 1550140

  •  MDL number MFCD00063691

  •  PubChem Substance ID 24891417

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Properties

Related Categories Chromogenic, Galactoside, Gene Reporter Substrates, Glycobiology, Inhibitors and Substrates,
grade   for molecular biology
InChI Key   LINMATFDVHBYOS-MBJXGIAVSA-N
sterility   non-sterile
assay   ≥98% (HPLC)
form   powder
solubility   DMF: soluble
suitability   suitable for substrate for β-galactosidase
storage temp.   −20°C

Description

General description

Bluo-Gal is a chromogenic substrate for β-galactosidase, used to determine the presence or absence of a cloned DNA insert in bacteria growing on agar plates. Bluo-Gal is designed to replace X-Gal in blue-white selection of recombinant bacterial colonies with the lac+ phenotype.

Application

Bluo-Gal is suitable for use in selection of recombinant bacterial colonies with the lac+ phenotype. Bluo-Gal is suitable for use as β-galactosidase substrate in the staining of skin tissues for electron microscopy analysis, Dictyostelium samples and in the staining of vena cava of adult mice.

Chromogenic substrate suitable for identification of lac+ bacterial colonies. An alternative to 5-bromo-4-chloro-3-indolyl β-D-galactopyranoside (X-Gal), producing a darker blue color.

Packaging

100, 500 mg in poly bottle

Principle

When Bluo-Gal is hydrolyzed by β-galactosidase, the resulting product will form a blue precipitate. Lac+ colonies grown in the presence of Bluo-Gal turn a deeper blue color more intense than X-Gal, allowing for easy differentiation between lac+ and lac- colonies.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Protocols & Articles

Articles

Blue-White Screening & Protocols for Colony Selection

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Molecular biology, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Peer-Reviewed Papers
15

References

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