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B300-05 Sigma-Aldrich

Bovine Coronary Artery Endothelial Cells: BCAEC (Cryovial)

  •  NACRES NA.81

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Properties

Related Categories Cell Culture, Endothelial Cells, Primary Cell Culture, Primary Cells
Quality Level   100
biological source   bovine coronary artery (USDA-inspected cattle)
packaging   pkg of 500,000 cells
growth mode   Adherent
karyotype   2n = 60
morphology   Endothelial
application(s)   cell culture | mammalian: suitable
relevant disease(s)   cardiovascular diseases
storage temp.   −196°C

Description

General description

BCAEC have been used to demonstrate that the stress hormone cortisol inhibits NO synthase and Ca2+ mobilization leading to decreased NO release by endothelial cells, resulting in increased arterial contraction and decreased blood flow (Rogers, 2002). Similar action mechanism were determined for cocaine effects on arteries (He, 2005). Additionally it was found that cocaine-induced apoptosis of endothelial cells is mediated by attenuation of NO production, down-regulation of Bcl-2 and translocation of Bac to the mitochondria, followed by release of cytochrome c and activation of caspase 9 and caspase 3 (He, 2000, 2001). BCAEC were also used to determine that diabetes-related vascular dysfunction is mediated by decreased NO production due to direct substrate competition between NO synthase and arginase whose activity is markedly increased in many conditions characterized by vascular dysfunction (Romero, 2008). BCAEC were also used to evaluate novel mesh-like materials for their suitability for biomedical applications (Nair, 2004).

Cell Line Origin

Artery

Application

cardiovascular function, angiogenesis, vascular biology, regulation of coronary blood flow, cardiac functions, effects of vaso-dilators, coagulation, fibrinolysis, vessel wall tone, cytokine production

Components

Bovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSO

Preparation Note

• 3rd passage, >500,000 cells in Bovine Endothelial Cell Growth Medium containing 10% FBS & 10% DMSO
• Can be cultured at least 16 doublings

Subculture Routine

Please refer to the BCAEC Culture Protocol.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

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Protocols & Articles

Protocols

Protocol: Bovine Coronary Artery Endothelial Cells Culture

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