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  • B3150 - Monoclonal Anti-Protein A−Biotin antibody produced in mouse

B3150 Sigma

Monoclonal Anti-Protein A−Biotin antibody produced in mouse

clone SPA-27, purified immunoglobulin, buffered aqueous solution

Synonym: Monoclonal Anti-Protein A



Related Categories Alphabetical Index, Antibodies, Miscellaneous, PR-PR, Primary Antibodies More...
biological source   mouse
antibody form   purified immunoglobulin
clone   SPA-27, monoclonal
form   buffered aqueous solution
application(s)   direct ELISA: 1:80,000-1:160,000
isotype   IgG1
conjugate   biotin conjugate
shipped in   dry ice
storage temp.   −20°C



protein A from Cowan I strain of Staphy­lococcus aureus.

Preparation Note

Prepared by conjugation of biotinamidocaproate N-hydroxysuccinimide ester to fractionated monoclonal anti-Protein A.


Specific for Protein A and shows no cross-reactivity with Protein G. Reacts with free Protein A or IgG bound Protein A and does not interfere with the Fc binding activity of Protein A.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% inactivated bovine serum albumin and 15 mM sodium azide.


Monoclonal Anti-Protein A-Biotin antibody produced in mouse may be used in ELISA at a working dilution of 1:80,000 - 1:160,000.

Biochem/physiol Actions

Protein A is an immunoglobulin-binding protein present on the surface of Staphylococcus aureus and is also secreted into the extracellular environment. It binds the Fc region of Igs and Fab regions of B cell receptors. The coupling of biotin to monoclonal Anti-Protein A antibody allows for the binding of various labels such as avidin or streptavidin.

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NONH for all modes of transport
WGK Germany 
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
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