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B3804 Sigma-Aldrich

BCIP®/NBT-Blue Liquid Substrate System for Membranes

alkaline phosphatase substrate

Synonym: 5-Bromo-4-chloro-3-indolyl phosphate/nitro blue tetrazolium liquid substrate system

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Description

Application

A ready-to-use buffered alkaline phosphatase substrate for use in immunoblotting. Produces an insoluble dark blue-purple end product. Not recommended for microwell (e.g., ELISA) or immunohistochemical applications.

Caution

Stable at least one year at 2-8 °C. This substrate is light-sensitive.

Legal Information

BCIP is a registered trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

Symbol 
GHS07  GHS07
Signal word 
Warning
Hazard statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

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Certificate of Origin

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Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Colorimetric Alkaline Phosphatase and Peroxidase Substrate Detection Systems

Nitroblue Tetrazolium (NBT) is used with the alkaline phosphatase substrate 5-Bromo- 4-Chloro-3-Indolyl Phosphate (BCIP) in western blotting and immunohistological staining procedures. These substrat...
BioFiles 2008, 3.4, 6.
Keywords: Enzyme-linked immunosorbent assay, Immunohistochemistry, Western blot

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