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B4261 Sigma-Aldrich

Biocytin

≥98% (TLC)

Synonym: Bct, Nε-(+)-Biotinyl-L-lysine

  • CAS Number 576-19-2

  • Empirical Formula (Hill Notation) C16H28N4O4S

  • Molecular Weight 372.48

  •  Beilstein/REAXYS Number 97197

  •  MDL number MFCD00077319

  •  PubChem Substance ID 24891762

  •  NACRES NA.26

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Properties

Related Categories A - H, Amino Acids, Biochemicals and Reagents, Modified Amino Acids
InChI Key   BAQMYDQNMFBZNA-MNXVOIDGSA-N
assay   ≥98% (TLC)
optical activity   [α]20/D +53°, c = 1.05% in 0.1 M NaOH(lit.)
mp   ~245 °C (dec.) (lit.)
storage temp.   −20°C

Description

Application

Biocytin is used as a substrate to study the specificity and kinetics of biotinidase(s), to measure biotinidase deficiency and as a model compound to study various uptake mechanisms of cells and tissues. Biocytin has been used to label neurons, medium spiny neurons and optic nerves.

Packaging

25, 50, 100, 250 mg in poly bottle

5 mg in glass bottle

Biochem/physiol Actions

Biocytin is an adduct of lysine and biotin. It is hydrolyzed by biotinidase, which acts on peptide-incorporated biotin or free biotin. Biocytin acts as a coenzyme.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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92210 Timestrip Plus 0 °C
06693 Timestrip Plus -20 °C

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