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B4500 Sigma-Aldrich

Nα-Benzoyl-L-arginine ethyl ester hydrochloride

trypsin substrate

Synonym: BAEE

  • CAS Number 2645-08-1

  • Empirical Formula (Hill Notation) C15H22N4O3 · HCl

  • Molecular Weight 342.82

  •  Beilstein Registry Number 3781694

  •  EC Number 220-157-0

  •  MDL number MFCD00012579

  •  PubChem Substance ID 24891777

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Description

Packaging

10, 25 g in poly bottle

Substrates

The prototype substrate for trypsin.

Application

• N

• α-Benzoyl-L-arginine ethyl ester hydrochloride has been used as a substrate:
• in peptidyl arginine deiminase (PPAD) assay in P. gingivalis vesicles
• for assaying proteolytic activity of enzymatic extracts of papaya plant parts
• in trypsin activity assay of the tail tendon fascicles from rat

General description

Nα-Benzoyl-L-arginine ethyl ester hydrochloride is a substrate for trypsin. It is a nonprotein substrate used in spectroscopic assays.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles

Protocols

Enzymatic Assay of Trypsin Inhibitor

3.2 Unit Definition – One Trypsin BAEE unit will produce a ΔA253nm of 0.001 per minute with BAEE as substrate at pH 7.6 at 25ºC in a reaction volume of 3.2 ml.

Enzymatic Assay of Trypsinogen

This scope of this procedure applies to products that have a specification for the enzymatic activity of trypsinogen.
Keywords: Enzyme activity, Extinction coefficient

Procedure for Enzymatic Assay of Trypsin (EC 3.4.21.4)

This procedure is for determining Trypsin activity using Nα-Benzoyl-L-arginine ethyl ester (BAEE) as the substrate. The procedure is a continuous spectrophotometric rate determination (A253, Light pa...

Peer-Reviewed Papers
15

References

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