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B4639 Sigma-Aldrich

Biotin

powder, BioReagent, suitable for cell culture, suitable for insect cell culture, suitable for plant cell culture, ≥99%

Synonym: D-Biotin, Bios II, Coenzyme R, Vitamin B7, Vitamin H

  • CAS Number 58-85-5

  • Empirical Formula (Hill Notation) C10H16N2O3S

  • Molecular Weight 244.31

  •  Beilstein/REAXYS Number 86838

  •  EC Number 200-399-3

  •  MDL number MFCD00005541

  •  eCl@ss 34058011

  •  PubChem Substance ID 24891795

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Properties

Related Categories Amino Acids and Vitamins, Biochemicals and Reagents, Carbohydrates, Carbohydrates A to Z, Carbohydrates A-C,
InChI Key   YBJHBAHKTGYVGT-ZKWXMUAHSA-N
product line   BioReagent
assay   ≥99%
form   powder
mol wt   Mw 244.31 g/mol
application(s)   cell culture | insect: suitable
  cell culture | plant: suitable
color   white to off-white
mp   231-233 °C (lit.)
solubility   H2O: 0.2 mg/mL (Solubility increases with addition of 1 N NaOH.)
suitability   suitable for cell culture
  suitable for insect cell culture
  suitable for plant cell culture
storage temp.   2-8°C

Description

General description

Biotin is also called as B complex vitamin. It is a bicyclic compound, which has a molecular weight of 244.31 Da. Biotin serves as an important cofactor for mammalian carboxylases. It is found within the cells in free and bound pools.

Application

Biotin has been used:
• as a M199 media supplement in in vitro culture system
• as a component of SATO serum free medium for the isolation of cortical oligodendrocyte precursors (OPCs)
• in biochemical precursors culture for de novo fatty acid synthesis
• as a component in aggregate culture media

Packaging

1, 5 g in poly bottle

100, 500 mg in poly bottle

Biochem/physiol Actions

Essential vitamin that is important for amino acid and energy metabolism, and fatty acid synthesis. It is a prosthetic group in four mammalian carboxylase families and facilitates the binding and transfer of carbon dioxide.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
1
RTECS 
XJ9088200

Documents

Certificate of Analysis

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Certificate of Origin

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Cell Culture Guide

Milli-Q® Water Purification Solutions
Protocols & Articles

Related Content

Biotin in Cell Culture

Importance and uses of biotin in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures
Keywords: Asymmetric synthesis, Biotinylations, Cell culture, Citric Acid Cycle, Culture media, Degradations, Metabolism, Oxidations, Transcription, Vitamins

Cell Culture Troubleshooting Guide

Cell culture has become one of the most fundamental techniques for modeling biological systems, and is of increasing importance in the biotechnology and pharmaceutical sectors as well as an essential...
Keywords: Antibiotics, Apoptosis, Cell attachment, Cell culture, Cryopreservation, Filtration, Growth factors, Pharmaceutical, Phase transitions

Peer-Reviewed Papers
15

References

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08168 Timestrip Plus 8 °C
B0301 Biotin, meets USP testing specifications

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