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B4882 Sigma-Aldrich

Bromelain from pineapple stem

≥3 units/mg protein

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Properties

Related Categories 3.4.x.x Peptidases, 3.x.x.x Hydrolases, Biochemicals and Reagents, Bromelain, Enzyme Class Index,
Quality Level   200
form   lyophilized powder
composition   Protein, ≥30% biuret
storage temp.   −20°C

Description

Application

Bromelain from pineapple stem has been used:
• to evaluate the aqueous micellar two-phase systems composed of ionic liquids as co-surfactants
• as a positive control in sodium dodecyl sulfate (SDS) / polyacrylamide gel electrophoresis (PAGE) and in cells proliferation assay
• to study its potential analgesic effect and to evaluate its hydrolyzingeffects on proenkephalin (PENK)-derived peptides containing enkephalin sequences

Bromelain may be used to inhibit the biosysnthesis of proinflammatory prostaglandins. It may be used to reduce clotting efficiency. Bromelain, from pineapple stem, has been used to make enzymatic hydrolysates of honeybee-collected pollen .

Biochem/physiol Actions

Bromelain is a cysteine endopeptidase with broad specificity for cleavage of proteins. Bromelain may be from a stem or piece of fruit. Product B4882 is from pineapple stem. Product P3169 (pGlu-Phe-Leu-p-nitroanilide) may be used as a chromogenic substrate for the assay of bromelain.

Unit Definition

One unit will release 1.0 micromole of p-nitrophenol from N-alpha-CBZ-L-Lysine p-nitrophenyl ester per minute at pH 4.6 at 25 °C. One old titrimetric unit (pH 4.5 at 45 °C) is equivalent to approximately 1.7 new units (pH 4.6 at 25 °C).

Preparation Note

The product may be suspended in acetate buffer, pH 4.5 at 1 mg/mL concentration, yielding a hazy, off-white suspension.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 1
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles

Articles

HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column

From our library of Articles, Sigma-Aldrich presents HPLC Analysis of Bromelain on TSKgel® BioAssist® S vs. Competitive S Column
Keywords: Chromatography, High performance liquid chromatography

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Peer-Reviewed Papers
15

References

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inhibitor

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E3132 E-64, protease inhibitor

substrate

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P3169 pGlu-Phe-Leu p-nitroanilide, protease substrate
C3637 Z-L-Lys-ONp hydrochloride

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