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  • B5002 - 5-Bromo-2′-deoxyuridine

B5002 Sigma


≥99% (HPLC)

Synonym: 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR



Frequently Asked Questions

Frequently Asked Questions are available for this Product.

Biochem/physiol Actions

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.


1, 5, 100 g in glass bottle

100, 250, 500 mg in glass bottle


5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

Features and Benefits

This compound is also offered as part of Sigma′s Library of Pharmacologically Active Compounds (LOPAC®1280), a biologically annotated collection of high-quality, ready-to-screen compounds. Click here to learn more.

Legal Information

LOPAC is a registered trademark of Sigma-Aldrich Co. LLC

Price and Availability

LC-MS Grade Solvents and Reagents

Flow Cytometry

Milli-Q® Water Purification Solutions
Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

How does BrdU, Product B5002, work as a Thymidine analog?
BrdU replaces Thymidine during the S-phase of cell division.  For more information on BrdU, click here for a Product Information Datasheet on Product No. B5002, 5-Bromo-2′-deoxyuridine.
What are the hazards associated with BrdU, Product B5002?
BrdU is a known carcinogen and caution should be exercised when handling.  Click on the MSDS hyperlink located on the product detail page for Product No. B5002, 5-Bromo-2′-deoxyuridine. 
How long do I treat cells with Product B5002, BrdU to incorporate it into the DNA?
Actively growing cells may be treated with 10 µm BrdU for 1 hour.
What can I use to detect BrdU, Product B5002 in vivo and in vitro?
For the immunohistochemical detection of BrdU, we recommend Product No. B2531, Monoclonal Anti-BrdU antibody produced in mouse.  Click here for the product detail webpage of this antibody.
What is the stability of Product B5002,  BrdU?
BrdU is stable as a powder, kept in the freezer and has a recommended re-test date of two years according to the Product Specification Sheet for Product No. B5002.  Please consult our Product Dating Information Statement for more information on re-test dated products.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
How do I get lot-specific information or a Certificate of Analysis?
A Certificate of Analysis is available by lot number and can be obtained through our Advanced Search Option: http://www.sigmaaldrich.com/catalog/AdvancedSearchPage.do
How do I find price and availability?
There are several ways to find pricing and availability for our products.  Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers. 
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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Protocols & Articles


Inhibition of Autoregulated TFG-ß

We studied autocrine transforming growth factor (TGF)beta signaling in kidney epithelium. Cultured proximal tubule cells showed regulated signaling that was high during log-phase growth, low during c...
Keywords: Growth factors

Peer-Reviewed Papers


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B8434 Anti-BrdU antibody, Mouse monoclonal, clone BU-33, purified from hybridoma cell culture
B2531 Monoclonal Anti-BrdU antibody produced in mouse, clone BU-33, ascites fluid, Immunohistology Grade

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