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  • B5002 - 5-Bromo-2′-deoxyuridine

B5002 Sigma-Aldrich


≥99% (HPLC)

Synonym: 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR

  • CAS Number 59-14-3

  • Empirical Formula (Hill Notation) C9H11BrN2O5

  • Molecular Weight 307.10

  •  Beilstein/REAXYS Number 30395

  •  EC Number 200-415-9

  •  MDL number MFCD00006529

  •  PubChem Substance ID 24278270

  •  NACRES NA.51



Related Categories Apoptosis and Cell Cycle, BRDU Cell Proliferation Assay Reagents, Biochemicals and Reagents, Cancer Research, Cell Biology,
assay   ≥99% (HPLC)
form   powder
mp   191-194 °C (dec.) (lit.)
solubility   DMSO: 50 mg/mL, clear, colorless to very faintly yellow
storage temp.   −20°C
SMILES string   OC[C@H]1O[C@H](C[C@@H]1O)N2C=C(Br)C(=O)NC2=O
InChI   1S/C9H11BrN2O5/c10-4-2-12(9(16)11-8(4)15)7-1-5(14)6(3-13)17-7/h2,5-7,13-14H,1,3H2,(H,11,15,16)/t5-,6+,7+/m0/s1



5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

5-Bromo-2′-deoxyuridine has been used:
• as a supplement to study the effect of electroconvulsive seizures on hippocampal neurogenesis
• as a supplement to study the loss-of-gene function in adult zebrafish heart
• in T lymphocyte proliferation assay{133]


1, 5, 100 g in glass bottle

100, 250, 500 mg in glass bottle

Biochem/physiol Actions

5-Bromo-2′-deoxyuridine (BrdU) is used to analyze cell proliferation, because of its facile incorporation into DNA during the S phase of the cell cycle. BrdU stimulates cellular differentiation and maturation in leukemia cell lines, while it inhibits differentiation of friend erythroleukemia cells. Studies of BrdU incorporation often subsequently use BrdU-specific antibodies with fluorescent tags, for detection of the incorporated BrdU, via such methods as flow cytometry or fluorescence microscopy.

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.

Features and Benefits

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Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

How does BrdU, Product B5002, work as a Thymidine analog?
BrdU replaces Thymidine during the S-phase of cell division.
What are the hazards associated with BrdU, Product B5002?
BrdU is a known carcinogen and caution should be exercised when handling.Click on the MSDShyperlink located on theproduct detail page for Product No. B5002, 5-Bromo-2´-deoxyuridine.
How long do I treat cells with Product B5002, BrdU to incorporate it into the DNA?
Actively growing cells may be treated with 10 μm BrdU for 1 hour.
What can I use to detect BrdU, Product B5002 in vivo and in vitro?
For the immunohistochemical detection of BrdU, we recommendProduct No.B2531, Monoclonal Anti-BrdU antibody produced in mouse.
What is the stability of Product B5002,BrdU?
BrdU is stable as a powder, kept in the freezer and has a recommended re-test date of two years according to the Product Specification Sheet for Product No. B5002. Please consult ourProduct Dating Information Statementfor more information on re-test dated products.
Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 or view local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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Protocols & Articles


Inhibition of Autoregulated TFG-ß

We studied autocrine transforming growth factor (TGF)beta signaling in kidney epithelium. Cultured proximal tubule cells showed regulated signaling that was high during log-phase growth, low during c...
Keywords: Growth factors

Live Cell Cycle Assay

Regulation of the cell cycle involves processes crucial to the survival of a cell, including the detection and repair of genetic damage as well as the prevention of uncontrolled cell division associa...
Kevin Su1, Nick Asbrock1, Vi Chu1, Tomas Kraus2
1MilliporeSigma, Temecula, CA; 2Institute of Organic Chemistry and Biochemistry AS CR, Czech Republic
Keywords: Building blocks, Cancer, Cell culture, Cell division, Cell proliferation, Centrifugation, Confocal microscopy, Culture media, Enzyme-linked immunosorbent assay, Flow cytometry, Genetic, Microscopy, Peptide synthesis

Peer-Reviewed Papers


Related Products

also commonly purchased with this product

Product #


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B8434 Anti-BrdU antibody, Mouse monoclonal, clone BU-33, purified from hybridoma cell culture
B2531 Monoclonal Anti-BrdU antibody produced in mouse, clone BU-33, ascites fluid, Immunohistology Grade

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