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  • B5050 - Monoclonal Anti-Brain-derived Neurotrophic Factor antibody produced in mouse

B5050 Sigma

Monoclonal Anti-Brain-derived Neurotrophic Factor antibody produced in mouse

clone 35928.11, purified immunoglobulin, lyophilized powder

Synonym: Anti-BDNF



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology,
species reactivity   human
application(s)   immunohistochemistry (formalin-fixed, paraffin-embedded sections): 8-25 μg/mL using human spinal cord
  western blot: 1-2 μg/mL
clone   35928.11, monoclonal
antibody form   purified immunoglobulin
form   lyophilized powder
isotype   IgG1
storage temp.   −20°C
Gene Information   human ... BDNF(627)
biological source   mouse
conjugate   unconjugated



Recombinant human BDNF, expressed in Sf 21 cells.

General description

The antibody shows ~5% cross-reactivity with recombinant human β−NGF and recombinant rat β−NGF.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline containing carbohydrates.

Preparation Note

Purified from ascites fluid using protein A.


Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunofluorescence (1 paper)

Biochem/physiol Actions

Brain derived neurotrophic factor (BDNF) belongs to a family of secreted proteins called neurotrophins that includes, nerve growth factor (NGF), neurotrophin-3 (NT3) and NT4/5. BDNF is expressed in the developing and the mature brain. The downstream pathways promoted by BDNF include PI3K, MAPK and JAK/STAT. The most important role of BDNF is the regulation of synaptic transmission and plasticity, protein synthesis and phosphorylation of local proteins.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  3
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Monoclonal and polyclonal primary antibodies are focused on cell biology, neurobiology and molecular biology. Secondary antibodies targeting multiple host’s IgG are conjugated to alkaline phosphatase...
Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

Peer-Reviewed Papers


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