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B5388 Sigma

Brilliant Cresyl Blue ALD

certified by the Biological Stain Commission

Synonym: Brilliant Blue C, Brilliant Cresyl blue

  • CAS Number 81029-05-2

  • Linear Formula C17H20N3OCl · 1/2ZnCl2

  • Molecular Weight 385.96

  •  Colour Index Number 51010

  •  Beilstein Registry Number 5690713

  •  EC Number 279-675-0

  •  MDL number MFCD00148901

  •  PubChem Substance ID 24891836

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Properties

Related Categories B, Cell Biology, Certified Stains & Dyes, Hematology and Histology, Stains & Dyes, A to Z,
grade   certified by the Biological Stain Commission
InChI Key   SIHASHFMDFXGSX-UHFFFAOYSA-J
composition   Dye content, ≥60%

Description

Packaging

10 g in glass bottle

Application

Brilliant Cresyl Blue ALD has been used for the selection of oocytes. It has also been used for the selection of cumulus-oocyte complexes (COCs).

Biochem/physiol Actions

Brilliant Cresyl Blue ALD is mainly used for the selection of oocytes. It helps in determining the activity of glucose-6-phosphate dehydrogenase (G6PD). This enzyme is generated in growing oocytes. However, oocytes which complete their growth have low levels of G6PD. Low G6PD activity results in blue coloration of the cytoplasm because brilliant cresyl blue is not reduced to a colourless compound. It is also used for the staining in reticulocytes, where the dye stains reticulin.

Suitability

Certified as a supravital stain for reticulocytes.

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
3

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions

Biomedical Applications
Protocols & Articles
Peer-Reviewed Papers
15

References

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