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B6024 Sigma-Aldrich

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside

tablet

Synonym: 5-Bromo-4-chloro-3-indolyl β-D-galactoside, BCIG, X-Gal

  • CAS Number 7240-90-6

  • Empirical Formula (Hill Notation) C14H15BrClNO6

  • Molecular Weight 408.63

  •  Beilstein/REAXYS Number 1402009

  •  EC Number 230-640-8

  •  MDL number MFCD00005666

  •  PubChem Substance ID 57653950

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Properties

Related Categories Chromogenic, Galactoside, Gene Reporter Substrates, Glycobiology, Inhibitors and Substrates,
InChI Key   OPIFSICVWOWJMJ-AEOCFKNESA-N
form   tablet
suitability   suitable for identification of lac+ bacterial colonies
storage temp.   −20°C

Description

General description

5-Bromo-4-chloro-3-indolyl β -D-galactopyranoside, commonly known as X-Gal, is a histochemical substrate for β-galactosidase.

Application

5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside has been used in the preparation of X-Gal stock solution to add it in LB agar plates for blue-white screening of transformants.

X-Gal is a chromogenic substrate for β-galactosidase that produces a rich blue color that can easily be detected visually over background. X-Gal is the substrate of choice for blue-white selection of recombinant bacterial colonies with the lac+ genotype.

Biochem/physiol Actions

X-gal is routinely used to detect transgene activity in situ.

Quantity

Contains 5 mg substrate per tablet

Reconstitution

A stock solution (100 mg/ml) is prepared by dissolving X-Gal in dimethylformamide and should be stored at −20°C and protected from light. Sterilization is not required.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Blue-White Screening & Protocols for Colony Selection

Blue-white screening is a rapid and efficient technique for the identification of recombinant bacteria. It relies on the activity of β-galactosidase, an enzyme occurring in E. coli, which cleaves lac...
Keywords: Antibiotics, Cloning, Culture media, Gene expression, Immunocytochemistry, Molecular biology, Peptide synthesis, Polymerase chain reaction, Recombination, Sterilizations, transformation

Peer-Reviewed Papers
15

References

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