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B6185 Sigma-Aldrich

Bionic Buffer, 10X Concentrate

for Northern and Southern blotting, solution

Synonym: Sodium Borate Electrophoresis Buffer

  •  NACRES NA.25



Related Categories Buffers / Buffer salts, Core Bioreagents, Life Science Reagents for DNA/RNA Electrophoresis, Research Essentials
Quality Level   200
grade   for molecular biology
form   solution
concentration   10 ×
impurities   DNase, RNase and nickase, none detected
pH   8.0-8.5 (20-25 °C)
storage temp.   room temp


General description

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for higher voltage runs, extremely rapid running times, and high-resolution band separation.


Suitable for:
• Use as running buffer for nucleic acid electrophoresis, which may be part of Northern and Southern blot hybridization procedures
• Use with TBA and TAE gels
• Preparation of agarose gels

Features and Benefits

• Faster run times
• Sharper bands
• Easy to dilute and use
• Flexible use with a variety of gel types


The 4 L and 10 L sizes are supplied in dispensers with a spigot.


Test electrophoresis chambers to determine highest voltage. Voltages beyond the recommended range for your apparatus may result in blown fuses or damage to your apparatus.

Legal Information

Bionic is a trademark of Sigma-Aldrich Co. LLC

Safety & Documentation

Safety Information

NONH for all modes of transport
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Single- & multi-channel pipettes from BRAND
Protocols & Articles


Bionic™ Buffer FAQs

From our library of Articles, Sigma-Aldrich presents Bionic™ Buffer FAQs
Keywords: Cloning, Electrophoresis, Polymerase chain reaction, Purification

Intro to Southern & Northern Blotting | Northern & Southern Blot Protocols

The transfer of macromolecules such as nucleic acids and proteins to solid-phase membranous support is known as blotting. Fragments of DNA and RNA molecules separated by gel electrophoresis are trans...
Keywords: AGE, Buffers, Digestions, Electrophoresis, Filtration, Gel electrophoresis, Nucleic acid hybridization, Precipitation, Purification, Scintillation, Separation, Sterilizations

Nucleic Acid Electrophoresis Protocols & Introduction

Introduction Agarose gel electrophoresis for DNA Agarose gel electrophoresis for RNA Polyacrylamide gel electrophoresis for DNA Reference
Keywords: AGE, Buffers, Degradations, Electrophoresis, Environmental, Gel electrophoresis, PAGE, Purification, Separation, Size-exclusion chromatography

Related Content

Bionic™ Buffer Data

Bionic Buffer is a unique alternative to traditional TBE (TRIS borate-EDTA) and TAE (TRIS acetate-EDTA) electrophoresis buffers. Bionic Buffer allows for:
Keywords: Buffers, Electrophoresis, Polymerase chain reaction, Separation

Peer-Reviewed Papers


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GERPN3005 ECL Direct Nucleic Acid, Cytiva, RPN3005, pack of 1 ea
D9156 Deoxyribonucleic acid, single stranded from salmon testes, For hybridization

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