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B6775 Sigma-Aldrich

Monoclonal Anti-Human IgG1−Biotin antibody produced in mouse

clone 8c/6-39, purified immunoglobulin, buffered aqueous solution

Synonym: Anti Human IgG1 - Monoclonal Anti-Human IgG1-Biotin antibody produced in mouse, Anti Human Igg1, Monoclonal Anti-Human IgG1 (Fc specific)

  •  NACRES NA.46

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Properties

Related Categories Alphabetical Index, Antibodies, Biotin Antibodies, HU-HZ, Human IgG Secondary Antibodies and Conjugates,
conjugate   biotin conjugate
clone   8c/6-39, monoclonal
biological source   mouse
application(s)   direct ELISA: 1:1,000
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   purified immunoglobulin
isotype   IgG2a
Quality Level   200
antibody product type   secondary antibodies

Description

General description

Human IgGs are glycoprotein antibodies that contain two equivalent light chains and a pair of identical heavy chains. IgGs have four distinct isoforms, ranging from IgG1 to IgG4. These antibodies regulate immunological responses to allergy and pathogenic infections. IgGs have also been implicated in complement fixation and autoimmune disorders
Monoclonal Anti-Human IgG1-Biotin antibody is specific for an epitope in the Fc region of the human IgG1. The product does not react with human IgG2, IgG3, or IgG4. This antibody clone (HP6019 clone) has been recognized as a useful human IgG1 specificity standard in the WHO/IUIS study .

The antibody is specific for the Fc portion of human IgG1 and is non-reactive with other IgG subclasses. This clone, also described as HP-6091, was found to exhibit a valuable profile of reactivity and specificity for human IgG1 by the IUIS/WHO study.

Immunogen

Fc fragment of myeloma Human IgG

Application

Monoclonal Anti-Human IgG1-Biotin antibody is suitable for use in immunoblot. The product can also be used for direct ELISA (1:1,000).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 1% bovine serum albumin and 15 mM sodium azide

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 2
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

How to Choose a Secondary Antibody

The following information is provided to help you decide which secondary antibody may be best for your particular application.
Keywords: Amplification, Enzyme-linked immunosorbent assay, Flow cytometry, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Western blot

Secondary Antibodies, Conjugates and Kits

Secondary antibodies are polyclonal or monoclonal antibodies that bind to primary antibodies or antibody fragments, such as the Fc or Fab regions. They are typically labeled with probes that make the...
Keywords: Absorption, Adsorption, Amplification, Bacterial conjugations, Cancer, Digestions, Electrophoresis, Enzyme-linked immunosorbent assay, Flow cytometry, Gel electrophoresis, Gene expression, Hormones, Immobilization, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Immunology, Immunoprecipitation, Infrared spectroscopy, Microbiology, Microscopy, Purification, Vitamins, Western blot

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Peer-Reviewed Papers
15

References

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