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B8416 Sigma-Aldrich

Bleomycin sulfate from Streptomyces verticillus

1.5-2.0 units/mg solid, BioReagent, suitable for cell culture

Synonym: Blenoxane, Bleo, Blexane

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Properties

Related Categories Antibiotics, Antibiotics A to Z, Antibiotics by Application, Antibiotics by Mechanism of Action, Antifungal,
InChI Key   OOXTWFJZZAJGKA-CNLAFNBISA-N
product line   BioReagent
form   crystalline
application(s)   cell culture | mammalian: suitable
color   white
solubility   H2O: 20 mg/mL, clear, colorless
cation traces   Cu: ≤0.10%
suitability   suitable for cell culture
Mode of action   DNA synthesis | interferes
antibiotic activity spectrum   fungi
storage temp.   2-8°C

Description

Components

This product is a mixture of glycopeptides antibiotics containing Bleomycin A2 (70%) and B2(30%). Bleomycins differ from each other in the terminal amine and show varying biological activity.

Application

Bleomycin Sulfate is an atineoplastic antibiotic isolated from Streptomyces verticillus. It is recommended for use as a cell culture agent at 10-100 μg/mL.

Biochem/physiol Actions

Bleomycin sulfate binds to DNA, causes ssDNA scission at specific base sequences and inhibits DNA synthesis. This inhibitory action requires bleomycin to bind oxygen and a metal ion. It can also cleave RNA, to a lesser degree but more selectively. It acts as an inducer and regulator of apoptosis and inhibits tumor angiogenesis.

Caution

This product should be stored at 2-8°C.

Packaging

15 units in serum bottle

Preparation Note

The product is soluble in water in water at 20 mg/mL and is active for several days. A solution of 2 units/mL in 0.1 M neutral potassium phosphate should be used within 14 days. A solution is active for three months at 3 units/mL in normal saline.

General description

Chemical structure: glycopeptide

Other Notes

Keep container tightly closed in a dry and well-ventilated place, strongly hygroscopic

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
RIDADR 
NONH for all modes of transport
WGK Germany 
3
RTECS 
EC5991990

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Cell Culture Guide
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Peer-Reviewed Papers
15

References

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