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B8438 Sigma-Aldrich

Alcian Blue solution

1% in 3% acetic acid, pH 2.5

Synonym: Alcian Blue 8XG solution

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Properties

Related Categories A, Cell Biology, Hematology and Histology, Routine Histology Stains, Stains & Dyes, A to Z,
concentration   1% in 3% acetic acid, pH 2.5

Description

Packaging

250, 500 mL in glass bottle

Application

Alcian Blue solution has been used in the histological staining of acidic glycosaminoglycans (GAGs; mucopolysaccharides) that are carboxylated or sulfated. Sulfated glycosaminoglycans appear to be the preferred substrate for alcian blue at pH 2.5. Alcian blue has been used to stain hyaluronan (HA), a nonsulfated GAG, but the intensity of the staining of HA is affected by the method of tissue fixation used. Alcian blue staining of GAGs in tissue may be modified through the addition of an electrolyte such as magnesium chloride.

Biochem/physiol Actions

Alcian Blue is a cationic dye. It forms insoluble complexes with acidic glycosaminoglycans, thereby aiding in the quantitative determination of glycosaminoglycans. It is also used for the staining of bone and cartilage.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
2

Documents

Certificate of Analysis

Certificate of Origin


Milli-Q® Water Purification Solutions
Protocols & Articles
Peer-Reviewed Papers
15

References

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