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B8894 Sigma-Aldrich

Albumin, Acetylated from bovine serum

protease free, for molecular biology

Synonym: Ac-BSA, Acetyl BSA, Acetylated BSA

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Properties

Related Categories Albumin, Albumins by Application, Biochemicals, Biochemicals and Reagents, Blocking Reagents for Hybridization,
Quality Level   200
biological source   bovine
grade   for molecular biology
product line   BioReagent
form   solution
purified by   heat shock fractionation
contains   does not contain stabilizer or preservative
origin   USA origin
concentration   20 mg/mL in H2O
application(s)   FISH: suitable
impurities   HIV I and HIVII, HCV and HBsAg, tested negative
foreign activity   DNase free
  DNase, RNase, NICKase and protease, none detected
  NICKase free
  Protease free
  RNase free
storage temp.   −20°C
Gene Information   bovine ... ALB(280717)

Description

General description

Serum albumin is the most extensive plasma protein in the circulatory system. It is produced within the liver and exported as a non-glycosylated protein to the blood plasma. The molecular weight of native serum albumin is around 66 kDa. Native serum albumin is a negatively charged protein.

Acetylation of bovine serum albumin particularly modifies lysine (Lys) residues with elevated frequency, with lesser degrees of modification of serine (Ser) and threonine (Thr) residues. MALDI-TOF mass spectrometry analysis of acetylated BSA has indicated an increased MW of about 3000, compared to native BSA (Ostatná, V. et al., J. Electroanalytical Chem., 821, 97-103 (2018)).. This acetylation process also derivatizes similar amino acid residues in any trace contaminating nucleases in the BSA, to inactivate that nuclease activity. This makes acetylated BSA useful in molecular biology applications where trace nuclease activity must be minimized.

Application

Select the right albumin for your application with the Albumin Selection Guide

Acetylated BSA has been used as a component of the reaction mixture for adapter ligation. In addition, this B8894 acetylated BSA product has been used in other applications, such as:
• In cDNA library preparation (Hafner, M. et al., RNA, 17(9), 1697-1712 (2011))
• As a blocking agent with streptavidin beads used for capture of biotinylated RNA (Winz, M.-L. et al., Nat. Protocol., 12(1), 122-149 (2017))

Acetylated to inactivate nucleases commonly found in BSA. Acetylated BSA should not be used as a protein standard since acetylation of tyrosine residues prevents color development in the Lowry assay and other similar protein determinations.

Biochem/physiol Actions

Native serum albumin plays a major role in maintaining the pH and osmotic pressure of the blood. It is also involved in transporting a diverse range of endogenous and exogenous metabolites, such as amino acids, fatty acids, drugs, steroids and metal ions. Native serum albumin can have a role in modulating the levels of Ca2+ and Mg2+ metals in blood.

Features and Benefits

This acetylated BSA product has been tested for the following properties:
• DNase-free / Exonuclease-free
• Nickase-free / Endonuclease-free
• Protease-free
• RNase-free

Safety & Documentation

Safety Information

Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
WGK 3
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable

Documents

Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles
Peer-Reviewed Papers
15

References

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