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  • B9285 - 5-Bromo-2′-deoxyuridine

B9285 Sigma


BioUltra, ≥99%

Synonym: 5-BrdU, 5-Bromo-1-(2-deoxy-β-D-ribofuranosyl)uracil, 5-Bromouracil deoxyriboside, BUdR



Related Categories BRDU Cell Proliferation Assay Reagents, Biochemicals and Reagents, Cell Biology, Core Bioreagents, Nucleosides and Nucleotides,
product line   BioUltra
assay   ≥99%
impurities   ≤0.005% Phosphorus (P)
  ≤0.1% Insoluble matter
ign. residue   ≤0.1%
mp   191-194 °C (dec.)(lit.)
solubility   NH4OH: 0.1 M at 20 °C, clear, colorless
cation traces   Al: ≤0.0005%
  Ca: ≤0.01%
  Cu: ≤0.0005%
  Fe: ≤0.0005%
  K: ≤0.005%
  Mg: ≤0.0005%
  NH4+: ≤0.05%
  Na: ≤0.05%
  Pb: ≤0.001%
  Zn: ≤0.0005%
storage temp.   −20°C


Biochem/physiol Actions

Thymidine analog used as a mutagen in genetic research. Selectively incorporated into cellular DNA during S-phase.


1 g in poly bottle

50, 250 mg in poly bottle


5-Bromo-2′-deoxyuridine (5-BrdU) is a thymidine analogue which is incorporated into DNA. 5-BrdU is routinely and extensively used to measure DNA synthesis and to label dividing cells. Consequently 5-BrdU is used to study cell signaling and other processes that induce cell proliferation.

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Safety & Documentation

Safety Information

GHS08  GHS08
Signal word 
Hazard statements 
Precautionary statements 
NONH for all modes of transport
WGK Germany 

Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis. These documents are located on the product detail page under Useful Links & Tools. Click on the following link to search for a Certificate of Analysis. Please click the following link to see the details on our Product Dating Information.
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS. To access the shipping information for this material, use the link on the product detail page for the product, or search here. 
Can Product B9285, 5-Bromo-2′-deoxyuridine, be injected into animals?
Sigma has not injected animals with solutions of this compound, so we do not have a stepwise procedure for you.  However, this application has been reported in the literature.  Please see the cited references below, which you may find useful. Several references cite the use of Product No. B9285 in their protocols. 1. Yin, X-X, et al., Expression of active caspase-3 in mitotic and postmitotic cells of the rat forebrain. J. Comp. Neurol., 433(1), 4-22 (2001).  Injected into mice at 50 mg/kg. 2. Dackor, R.T., et al., Hydrops Fetalis, Cardiovascular Defects, and Embryonic Lethality in Mice Lacking the Calcitonin Receptor-Like Receptor Gene. Mol. Cell. Biol., 26(7), 2511-2518 (2006).  Injected into mice at 100 mg/kg. 3. Farris, S.M., et al., Larval and Pupal Development of the Mushroom Bodies in the Honey Bee, Apis mellifera. J. Comp. Neurol., 414, 97-113 (1999).  A 25 mg/mL solution was injected into bee larvae, prepupae, and pupae. 4. Gaiano, N., et al., Radial Glial Identity Is Promoted by Notch1 Signaling in the Murine Forebrain. Neuron, 26(2), 395-404 (2000).  A 20 mg/mL solution was injected into mice.
For how long is Product B9285, 5-Bromo-2′-deoxyuridine, stable in solution?
5-Bromo-2′-deoxyuridine is stable in solution for 6 months when stored -20°C.
In what solvents is Product B9285, 5-Bromo-2′-deoxyuridine, soluble?
Solubility information for 5-bromo-2′-deoxyuridine can be found on the product information sheet. Here is the link to that document: http://www.sigmaaldrich.com/sigma/product%20information%20sheet/b9285pis.pdf
What is the shelf life of Product No. B9285 (5-bromo-2′-deoxyuridine)?
Sigma-Aldrich does not assign an expiration date to this product; it is guaranteed for one year from the date of shipment.
My question is not addressed here, how can I contact Technical Service for assistance?
Use the option to the right to "Ask a Question" by email of a Technical Service Scientist.
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