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  • B9428 - Monoclonal Anti-Bin1 antibody produced in mouse

B9428 Sigma

Monoclonal Anti-Bin1 antibody produced in mouse

clone 99D, ascites fluid

Synonym: Anti-Amphiphysin II, Anti-SH3P9, Anti-ampl



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Cell Cycle, Antibodies to Nuclear Proteins,
species reactivity   mouse, human, rat, avian, rabbit
application(s)   flow cytometry: suitable
  immunocytochemistry: suitable using nucleoplasm, subnuclear punctate compartment, and cytoplasm
  immunohistochemistry (frozen sections): suitable
  immunoprecipitation (IP): suitable
  indirect ELISA: suitable
  western blot: 1:50,000 using a rat brain cytosol preparation
clone   99D, monoclonal
antibody form   ascites fluid
isotype   IgG2b
mol wt   antigen mol wt 65-75 kDa
contains   15 mM sodium azide
shipped in   dry ice
storage temp.   −20°C
Gene Information   human ... BIN1(274)
mouse ... Bin1(30948)
rat ... Bin1(117028)
biological source   mouse
conjugate   unconjugated



recombinant polypeptide containing amino acids 189-398 of human Bin1.


The antibody reacts specifically with a 33-residue segment of the myc-binding domain (MBD, amino acids 323-356) of the human Bin1 molecule. It does not react with the Bin1-related 45 kDa polypeptide.

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RIDADR  NONH for all modes of transport
WGK Germany  3
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Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Keywords: Amplification, Buffers, Cell biology, Enzyme-linked immunosorbent assay, Immunofluorescence, Immunohistochemistry, Inflammation, Molecular biology, Phosphorylations, Purification, Western blot

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