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  • B9804 - Anti-Bcl-2 antibody, Mouse monoclonal

B9804 Sigma

Anti-Bcl-2 antibody, Mouse monoclonal

clone 10C4, purified from hybridoma cell culture



Related Categories Alphabetical Index, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology, Antibodies for Hematopoietic Stem Cells,
species reactivity   mouse, rat
application(s)   microarray: suitable
  western blot: 2-10 μg/mL using rat osteosarcoma ROS cell extract
clone   10C4, monoclonal
concentration   ~2 mg/mL
antibody form   purified immunoglobulin
form   buffered aqueous solution
isotype   IgG1
mol wt   antigen mol wt 26 kDa
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Bcl2(12043)
rat ... Bcl2(24224)
biological source   mouse
conjugate   unconjugated



synthetic peptide corresponding to amino acids 61-76 of the mouse Bcl-2 sequence, conjugated to KLH.

Physical form

Solution in phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.


The epitope recognized by the antibody resides within amino acids 61-76 of the mouse Bcl-2 protein.

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Safety Information

RIDADR  NONH for all modes of transport
WGK Germany  2
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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