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BCK647-IV-FC-S Sigma-Aldrich

In Vivo EdU Flow Cytometry Kit 647

sufficient for 50 assays

  •  NACRES NA.22

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Properties

Related Categories Cell Biology, EdU Cell Proliferation Detection Kits, In Vivo Kits More...
fluorescence   λex 641 nm; λem 663 nm
shipped in   wet ice
storage temp.   2-8°C

Description

General description

The Baseclick EdU In Vivo Kits provide a superior alternative to BrdU and [3H]thymidine assays for detection of cell proliferation in whole animals, showing no toxic effects. EdU (5-ethynyl-2’-deoxyuridine) is a nucleoside analog to thymidine and is incorporated into DNA during active DNA synthesis. In contrast to BrdU assays, the EdU In Vivo Assays are not antibody based and therefore do not require DNA denaturation for detection of the incorporated nucleoside. Instead, the EdU In Vivo Kits utilize click chemistry for detection in a variety of dye fluorescent readouts. Furthermore, the streamlined detection protocol reduces both the total number of steps and significantly decreases the total amount of time. The simple click chemistry detection procedure is complete within 30 minutes and is compatible with multiplexing for content and context-rich results.The in vivo kits are designed in a sense that you combine one of the three baseclick EdU cell proliferation kits such as:

1. Imaging kit (BCK-IV-IM)
2. Flow cytometry kit (BCK-IV-FC)
3. High throughput screening – HTS kit (BCK-IV-HTS)

You can select the above kits with the dye of choice and the right EdU content for your animal model.

Depending on your animal model or the number of animals to be tested you can choose between three kit sizes S, M, and L with increasing EdU content, as indicated in the following table.

Kit Size Content of EdU
S 50 mg
M 500 mg
L 1000 mg

Application

in Vivo, Flow Cytometry

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport

Documents

Certificate of Analysis (COA)

Please Enter a Lot Number
Protocols & Articles

Articles

Baseclick EdU Cell Proliferation Detection Kits: Improve Data Quality and Save Time

Experimental studies measuring cell proliferation have had implications in cancer biology, immunology, cell biology, and developmental biology.  Various methods for monitoring cell proliferation have...
Keywords: Cancer, Catalysis, Cell biology, Cell proliferation, Click chemistry, DNA replication, Degradations, Flow cytometry, Immunology, Immunostaining, Indicators, Microscopy, Nucleic acid denaturation

Peer-Reviewed Papers
15

References

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