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C0543 Sigma-Aldrich

Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane

Type IV (Miller), lyophilized powder (from 0.2 μm-filtered solution), BioReagent, suitable for cell culture

Synonym: EHS-collagen



Related Categories Adhesion, Attachment, and Matrix Factors for Stem Cell Expansion, Attachment Factors, Attachments Factors, Biochemicals and Reagents, Cell Biology,
Quality Level   200
biological source   mouse (Engelbreth-Holm-Swarm murine sarcoma basement membrane)
sterility   non-sterile
type   Type IV (Miller)
product line   BioReagent
form   lyophilized powder (from 0.2 μm-filtered solution)
packaging   glass bottle of 1 vial
application(s)   cell culture | mammalian: suitable
surface coverage   6‑10 μg/cm2
solubility   0.25% acetic acid: 0.5-2.0 mg/mL (Keep at 2-8 °C for several hours, occasionally swirling.)
shipped in   dry ice
storage temp.   −20°C
Gene Information   mouse ... Col4a1(12826), Col4a2(12827), Col4a3(12828), Col4a5(12830), Col4a6(94216)


General description

The substrate derived from engelbreth-holm-swarm murine sarcoma is laminin rich basement membrane. It is commercially available as matrigel and cultrex. Matrigel is made of type 4 collagen, laminin, heparan sulfate proteoglycan and entactin obtained from engelbreth-holm-swarm murine sarcoma. Type 4 collagen is a major component of basement membrane.

Collagen is classified into a number of structurally and genetically distinct types. We use the nomenclature proposed by Bornstein and Traub. Do not confuse Sigma type designations with recognized collagen classification types.


Collagen from Engelbreth-Holm-Swarm murine sarcoma basement membrane has been used in adhesion assays.


1 vial in glass bottle

Biochem/physiol Actions

Collagen isolated from Engelbreth-Holm-Swarm murine sarcoma basement membrane helps in the development of 3D nodules. It is also used in 2D coatings.

Other Notes

Native collagen


0.75 mg/vial

Preparation Note

Chloroform treated.
Pepsin is not used in the preparation.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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