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C0615 Sigma-Aldrich

Cellulase from Trichoderma sp.

Green Alternative

powder, ≥5,000 units/g solid

Synonym: Onozuka RS

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Description

General description

Manufactured by Yakult

Sigma Life Science is committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has been enhanced for waste prevention when used in cellulosic ethanol research. For more information see the article in biofiles.

Application

Cellulases are enzymes that hydrolyze cellulose to glucose. Cellulase is used to study the development of occupational asthma in the detergent, pharmaceutical, baking, and enzyme production industries. Cellulase is added to detergents to improve cleansing properties. Cellulase from Sigma has been used to degrade cello-oligosaccharides into glucose while investigating the biodegradability of bioabsorbable bacterial cellulose (BBC).

Packaging

1 g in glass bottle

Biochem/physiol Actions

Cellulases are produced by the intestinal flagellates of termites which are important for cellulose digestion of their host termites . Cellulases have different microbial origins and therefore different protein sequences .

Unit Definition

One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37 °C (2 hr incubation time).

Preparation Note

Dissolves in sterile deionized (DI) water in the presence of 0.15 polyhexamethylene biguanide (PHMB) at 3 mg/mL concentration.

Other Notes

View more information on enzymes for complex carbohydrate analysis at www.sigma-aldrich.com/enzymeexplorer

Safety & Documentation

Safety Information

Symbol 
GHS08  GHS08
Signal word 
Danger
Hazard statements 
Precautionary statements 
Personal Protective Equipment 
RIDADR 
NONH for all modes of transport
WGK Germany 
1

Documents

Certificate of Analysis

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Certificate of Origin

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
How do I find price and availability?
There are several ways to find pricing and availability for our products.Once you log onto our website, you will find the price and availability displayed on the product detail page.You can contact any of our Customer Sales and Service offices to receive a quote. USA customers: 1-800-325-3010 orview local office numbers.
What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
Does product C0615, cellulase from Trichoderma viride, contain any sugar as a stabilizer?
No. Product C0615, cellulase from Trichoderma viride, does not contain any sugar stabilizer.
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Protocols & Articles

Protocols

Enzymatic Assay of Cellulase

3.12 Unit definition: One unit will liberate 1.0 μmole of glucose from cellulose in one hour at pH 5.0 at 37ºC (2 hour incubation time).
Keywords: Extinction coefficient

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Peer-Reviewed Papers
15

References

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