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C0678 Sigma-Aldrich

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse

clone VC1.1, ascites fluid, buffered aqueous solution

Synonym: Anti-CD57, Anti-N-CAM, Monoclonal Anti-HNK-1

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Properties

Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Cell Biology, Antibodies for Immune Cell Signaling and Blood,
conjugate   unconjugated
clone   VC1.1, monoclonal
biological source   mouse
application(s)   western blot: 1:4,000 using cerebral cortex extract
species reactivity   rat, human, feline, mouse
form   buffered aqueous solution
shipped in   dry ice
storage temp.   −20°C
antibody form   ascites fluid
isotype   IgM
antibody product type   primary antibodies
contains   15 mM sodium azide
UniProt accession no.   Q9P2W7
Gene Information   human ... B3GAT1(27087)
mouse ... B3gat1(76898)
rat ... B3gat1(117108)

Description

General description

Monoclonal Anti-HNK-1/N-CAM (CD57) (mouse IgM isotype) is derived from the VC1.1 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from immunized BALB/c mice. Human natural killer cell -1 (HNK-1), also referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP, is mapped to human chromosome 11q25. The encoded protein belongs to the glucuronyltransferase gene family. Neural cell-adhesion molecule (N-CAM) is expressed on all neuroepithelial cells from the time of neural induction in the early embryo. It is known that the sulfated carbohydrate HNK-1 epitope is carried by several surface molecules including a proportion of N-CAM isoforms.

Specificity

Recognizes the CD57/HNK-1 human myeloid cell associated surface glycoprotein. The epitope recognized is an N-linked carbohydrate which is present in a variety of glycoproteins and in some glycolipids. It is resistant to formalin fixation and paraffin embedding. VC1.1 antibody and the HNK-1 (Leu7) antibody inhibit the binding of each other. The antibody recognizes myelin associated glycoprotein in some species and a high molecular weight chondroitin sulphate proteoglycan.
5th Workshop: code no. NK 67

Immunogen

A homogenate of cat primary visual cortex (area 17)

Application

Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below.
Immunohistochemistry (1 paper)

Monoclonal Anti-HNK-1/N-CAM (CD57) antibody produced in mouse has been used:
•  as primary antibody for western blot analysis
•  immunocytochemistry
•  immunocytochemical
• immunohistological staining

Target description

CD57/HNK-1 is expressed on a subpopulation of 15-20% of peripheral blood mononuclear cells, about 60% of NK active cells and on a subset of T cells.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

HNK1 (human natural killer-1) is referred as B3GAT1, NK1, CD57, LEU7, NK-1, GLCATP and GLCUATP. It is a member of the glucuronyltransferase gene family. Human natural killer-1 (HNK1) carbohydrate epitope is expressed on various cell adhesion molecules in the nervous system and plays a role in cell-cell and cell-substrate interactions. HNK-1 epitope stabilizes GluR2 on neuronal surface membranes and regulates cell surface stability of GluR2 by modulating the interaction with N-cadherin. It is also plays a role in cell migration and synaptic plasticity. HNK-1 epitope has been associated with the risk of metastasis. It is observed to be highly expressed in melanoma cell lines.

Safety & Documentation

Safety Information

RIDADR 
NONH for all modes of transport
WGK Germany 
3
Protocols & Articles

Articles

Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy

Protocols

Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

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Peer-Reviewed Papers
15

References

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