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  • C0730 - Anti-CD137 antibody produced in goat

C0730 Sigma-Aldrich

Anti-CD137 antibody produced in goat

affinity isolated antibody, lyophilized powder

Synonym: Anti-4-1BB



Related Categories Alphabetical Index, Analytical Cytology, Antibodies, Antibodies for Apoptosis, Antibodies for Cell Biology,
conjugate   unconjugated
clone   polyclonal
biological source   goat
application(s)   capture ELISA: 0.2-0.8 μg/mL
  flow cytometry: 2.5 μg using 106 human T cells treated with PHA
  immunocytochemistry: 5-15 μg/mL
  western blot: 0.1 μg/mL
species reactivity   human
form   lyophilized powder
storage temp.   −20°C
antibody form   affinity isolated antibody
antibody product type   primary antibodies
impurities   <0.10 EU/μg endotoxin
UniProt accession no.   Q07011
Gene Information   human ... TNFRSF9(3604)


General description

CD137 (4-1BB) is a member of the tumor necrosis factor (TNF) receptor superfamily. It is a type I transmembrane glycoprotein expressed on the cell surface of activated splenic T cells and thymocytes.


mouse myeloma cell line NS0-derived recombinant human 41BB/TNFRSF9/CD137 Leu24Gln186


Anti-CD137 antibody produced in goat is suitable for the following applications:
• Agonist Activity: Measured by its ability to costimulate IFNg secretion by human T cells in the presence of anti-CD3. The ED50 for this effect is typically 5-20μg/mL.
• ELISA capture: It can be used as a capturing reagent in a human 4-1BB sandwich immunoassay in combination with biotinylated human 4-1BB detection antibody and recombinant human 4-1BB as the standard. The suggested coating concentration range is 0.2-0.8μg/ml. The recommended concentration of the detection antibody is 0.1-0.4μg/mL.
• Flow cytometry: 2.5μg/106 cells using Human T cells treated with PHA (phytohemagglutinin).
• Immunoblotting: A working concentration of 0.1μg/ml was determined using recombinant human CD137.
• Immunocytochemistry: 5-15μg/mL using immersion fixed human peripheral blood mononuclear cells.

Physical form

Lyophilized from a 0.2 μm filtered solution in phosphate buffered saline with 5% trehalose.


Reconstitute at 0.2 mg/mL in sterile PBS


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

CD137 in T lymphocytes helps to regulate activation, proliferation and apoptosis. CD137 and CD28 are co-stimulatory molecules of T cell activation. It plays an important role in regulating T-cell-dependent immune responses. The expression of CD137 correlates negatively with lymphocyte proliferation and induces cell death caused by mitogen overstimulation. In monocytes, CD137 induces activation, promotes adherence and helps in prolongs survival.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Protocols & Articles


Antibody Basics

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Western Blot Protocol | Immunoblotting Protocol

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