• USA Home
  • C0739 - Anti-phospho-Cortactin (pTyr421) antibody produced in rabbit

C0739 Sigma-Aldrich

Anti-phospho-Cortactin (pTyr421) antibody produced in rabbit

affinity isolated antibody, buffered aqueous glycerol solution



Related Categories Alphabetical Index, Antibodies, Antibodies for Cell Biology, Antibodies for Kinase/Phosphatase Biology, Antibodies to Actin-associated Proteins/Myosin,
conjugate   unconjugated
clone   polyclonal
biological source   rabbit
application(s)   immunocytochemistry: suitable
  western blot: 1:1,000 using 5HD47 cells
species reactivity   mouse
mol wt   antigen 80-85 kDa
form   buffered aqueous glycerol solution
shipped in   wet ice
storage temp.   −20°C
antibody form   affinity isolated antibody
Quality Level   200
antibody product type   primary antibodies
UniProt accession no.   Q60598
Gene Information   mouse ... Cttn(13043)


General description

Cortactin is encoded by the CTTN gene on chromosome 11 in humans. It is a cytoskeleton protein that facilitates assembly of cortical actin. It is expressed in most adherent cells and is a prominent substrate of protein-tyrosine kinase Src.


synthetic phosphopeptide derived from the region of mouse cortactin that is phosphorylated on tyrosine 421.


Anti-phospho-Cortactin (pTyr421) antibody produced in rabbit is suitable for immunocytochemistry and western blotting at a working dilution of 1:1,000 using 5HD47 cells.

Physical form

Solution in Dulbecco′s phosphate buffered saline (without Mg2+ and Ca2+), pH 7.3 (+/- 0.1), 50% glycerol with 1.0 mg/mL bovine serum albumin (IgG and protease free) and 0.05% sodium azide.


Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Biochem/physiol Actions

Cortactin is activated by phosphorylation of tyrosine kinases or serine/threonine kinases. It plays an important role in promoting lamellipodia formation, invadopodia formation, cell migration, and endocytosis. Cortactin regulates the interactions between components of adherens-type junctions and helps in organizing the cytoskeleton, cell adhesion structures of epithelia and carcinoma cells. During apoptosis, the encoded protein is degraded in a caspase-dependent manner. It acts as a regulator in invasion and migration of tumor cells. The overexpression of cortactin causes osteosarcoma carcinogenesis and along with FAK increases the risk of oral premalignant lesions.

Safety & Documentation

Safety Information

NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

Please Enter a Lot Number

Certificate of Origin (COO)

Please Enter a Lot Number
Protocols & Articles


Antibody Basics

Immunoglobulins (Igs) are produced by B lymphocytes and secreted into plasma. The Ig molecule in monomeric form is a glycoprotein with a molecular weight of approximately 150 kDa that is shaped more ...
Keywords: Affinity chromatography, Centrifugation, Chromatography, Digestions, Direct immunofluorescence, Gene expression, High performance liquid chromatography, Immunofluorescence, Ion Exchange, Microscopy, Precipitation, Purification, Rheumatology, Scanning electron microscopy


Western Blot Protocol | Immunoblotting Protocol

Western Blotting refers to the electrophoretic transfer of proteins from sodium dodecyl sulfate polyacrylamide gels to sheets of PVDF or nitrocellullose membrane, followed by immunodetection of prote...
Keywords: AGE, Buffers, Cell disruption, Detection methods, Detergents, Dialysis, Electroblotting, Electrophoresis, Enzyme activity, Gel electrophoresis, Immunoprecipitation, PAGE, Protein extraction, Purification, Sample preparations, Western blot

Related Content

Antibody Explorer | Buy Primary Antibodies & Secondary Antibodies

Primary, Secondary and Recombinant Monoclonal Antibodies Providing highly cited primary and secondary antibodies, we have you covered for your ELISA, western blot, immunohistochemistry or other assay...
Keywords: Alzheimer Disease, Cancer, Chromatin immunoprecipitation, Diagnostic, Enzyme-linked immunosorbent assay, Flow cytometry, Immunocytochemistry, Immunofluorescence, Immunohistochemistry, Neuroscience, Western blot

Peer-Reviewed Papers


Related Products

Technical Service:

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Bulk Ordering & Pricing:

Need larger quantities for your development, manufacturing or research applications?