C0875 Sigma-Aldrich


Used for the fluorometric determination of calcium and EDTA titration of calcium in the presence of magnesium.

Synonym: Bis[N,N−bis(carboxymethyl)aminomethyl]fluorescein, Fluorescein-bis(methyliminodiacetic acid), Fluorexon

  • CAS Number 154071-48-4

  • Empirical Formula (Hill Notation) C30H26N2O13

  • Molecular Weight 622.53

  •  Beilstein/REAXYS Number 8181068

  •  PubChem Substance ID 24892279

  •  NACRES NA.47



Related Categories Analytical Reagents, Analytical/Chromatography, Biochemicals and Reagents, C, Calcium Probes,
Quality Level   200
description   mixed isomers
form   powder
application(s)   hematology: suitable
  histology: suitable
  titration: suitable
solubility   NaOH: 50 mg/mL
fluorescence   λex 470 nm; λem 509 nm (pH 7.4)
Featured Industry   Diagnostic Assay Manufacturing
storage temp.   room temp
SMILES string   OC(=O)CN(CC(O)=O)Cc1cc2c(Oc3cc(O)c(CN(CC(O)=O)CC(O)=O)cc3C24OC(=O)c5ccccc45)cc1O


General description

Calcein is useful in fitting soft and hybrid contact lenses, along with its evaluation. It has a molecular weight of 710kDa and is generally preferred over fluorescein. Calcein is known to stain devitalized tissue. It is not preferred for highly hydrated soft lenses.


Calcein has been used:
• in whole cell staining
• in histomorphometry and micro-computed tomography
• to label active bone-forming surfaces


5, 10, 25 g in glass bottle

Biochem/physiol Actions

Calcein is a calcium-dependent fluorescent molecule. It is mainly required to study bone metabolism under in vivo conditions and helps in the staining of pit area under in vitro conditions. Calcein is used for the fluorometric determination of calcium and EDTA (ethylenediaminetetraacetic acid) titration of calcium.

Safety & Documentation

Safety Information

Personal Protective Equipment 
NONH for all modes of transport
WGK Germany 
Flash Point(F) 
Not applicable
Flash Point(C) 
Not applicable


Certificate of Analysis (COA)

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Certificate of Origin (COO)

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Frequently Asked Questions

Which document(s) contains shelf-life or expiration date information for a given product?
If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.
How do I get lot-specific information or a Certificate of Analysis?
The lot specific COA document can be found by entering the lot number above under the "Documents" section.
What are the excitation and emission wavelengths of Calcein, Product C0875?
Conn's Biological Stains, 10th Edition states excitation is at 494 nm in aqueous solutions at pH 9; the emission maximum is 517 nm. Depending upon the pH of the solution, slightly different values have been reported in the literature.
Is there a reference for usingCalcein, Product C0875, to study bone growth?
Clement-Lacroix, P., et al. Osteoblasts are a new target for prolactin: analysis of bone formation in prolactin receptor knockout mice. Endocrinology, 140, 96-105 (1999).
What is the extinction coefficient of Calcein, Product C0875?
For samples in 1 M sodium hydroxide: E1% = 1040 at 500-504 nm; E1% = 466 at 242-245 nm.
What is the solubility of Calcein, Product C0875?
Calcein is soluble at a concentration of 50 mg/mL in 1M sodium hydroxide, yielding clear orange to brown solution.
How do I find price and availability?
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What is the Department of Transportation shipping information for this product?
Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product.
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